2026-09-19T20:43:21Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/328812022-01-13T07:54:36Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
Ian W. Hunter.
Ball, Nathan B
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Massachusetts Institute of Technology. Department of Mechanical Engineering
2006-05-15T20:36:41Z
2006-05-15T20:36:41Z
2005
2005
http://hdl.handle.net/1721.1/32881
62589055
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005.
Includes bibliographical references (leaf 51).
A process known as Constant Denaturant Capillary Electrophoresis is used to separate mutant from wild-type DNA at fractions down to 10-7. A device known as an Ultra-high Throughput Mutational Spectrometer is being created to run 10,000 parallel channels of CDCE in order to correlate multiple point mutations in DNA with the diseases that they can cause, such as cancer. By separating the DNA in large populations, the underlying causes of such diseases can be identified. To successfully run CDCE, a high viscosity polymer gel must be loaded into each of the 10,000 channels, each of which are composed of an individual glass capillary with a 75 m inner diameter. A mechanism was designed and tested which loaded gel into 8 channels simultaneously. The mechanism was used to test the relationship between gel loading time in relation to varied pressure and capillary length, through 45 total runs, with 8 channels per run. The relationships were characterized, resulting in two equations that enable an accurate prediction of the fill time necessary to load 10,000 parallel channels simultaneously under varied conditions.
by Nathan B. Ball.
S.B.
73 leaves
2890463 bytes
2893211 bytes
application/pdf
application/pdf
eng
Massachusetts Institute of Technology
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http://dspace.mit.edu/handle/1721.1/7582
Mechanical Engineering.
Design and characterization of a gel loading mechanism for an ultra-high throughput mutational spectrometer
Thesis
application/pdf
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<Abstract>A process known as Constant Denaturant Capillary Electrophoresis is used to separate mutant from wild-type DNA at fractions down to 10-7. A device known as an Ultra-high Throughput Mutational Spectrometer is being created to run 10,000 parallel channels of CDCE in order to correlate multiple point mutations in DNA with the diseases that they can cause, such as cancer. By separating the DNA in large populations, the underlying causes of such diseases can be identified. To successfully run CDCE, a high viscosity polymer gel must be loaded into each of the 10,000 channels, each of which are composed of an individual glass capillary with a 75 m inner diameter. A mechanism was designed and tested which loaded gel into 8 channels simultaneously. The mechanism was used to test the relationship between gel loading time in relation to varied pressure and capillary length, through 45 total runs, with 8 channels per run. The relationships were characterized, resulting in two equations that enable an accurate prediction of the fill time necessary to load 10,000 parallel channels simultaneously under varied conditions.</Abstract>
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