2026-09-19T07:06:22Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/329362022-01-13T07:54:36Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
Todd Thorsen.
Hu, Jenny (Jenny Ezu)
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Massachusetts Institute of Technology. Department of Mechanical Engineering
2006-05-15T20:40:26Z
2006-05-15T20:40:26Z
2005
2005
http://hdl.handle.net/1721.1/32936
62783474
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005.
Includes bibliographical references (p. 51-53).
Two elastomeric microfluidic devices were designed for the purpose of conducting rapid, flow-based, multiplexed DNA hybridization. Experimental results showed that flowing hybridization assays could detect similar concentrations of labeled probe as standard stationary microarrays, but in 1/100h of the time, using 2% of the sample volume. An 8-channel device was used to spot glass slides with 64 hybridization assays and generate data supporting a theoretical model of DNA hybridization in both traditional stationary microarrays and flowing sample arrays. Larger devices were also used to create rrays of 96x96 spots on a single slide, demonstrating the scalability of the technology. Protocols were written and optimized for the use of both chips, allowing the technology to be distributed to collaborating labs for further development.
by Jenny Hu.
S.B.
60 p.
3815537 bytes
3817203 bytes
application/pdf
application/pdf
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
http://dspace.mit.edu/handle/1721.1/7582
Mechanical Engineering.
Characterization and optimization of PDMS microfluidic devices for rapid DNA hybridization
Thesis
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<Abstract>Two elastomeric microfluidic devices were designed for the purpose of conducting rapid, flow-based, multiplexed DNA hybridization. Experimental results showed that flowing hybridization assays could detect similar concentrations of labeled probe as standard stationary microarrays, but in 1/100h of the time, using 2% of the sample volume. An 8-channel device was used to spot glass slides with 64 hybridization assays and generate data supporting a theoretical model of DNA hybridization in both traditional stationary microarrays and flowing sample arrays. Larger devices were also used to create rrays of 96x96 spots on a single slide, demonstrating the scalability of the technology. Protocols were written and optimized for the use of both chips, allowing the technology to be distributed to collaborating labs for further development.</Abstract>
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