2026-09-19T16:56:44Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/339132022-01-13T07:54:36Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Ian W. Hunter.
Manoosingh, Sasha R. (Sasha Rianne), 1979-
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Massachusetts Institute of Technology. Department of Mechanical Engineering
2006-08-25T18:56:13Z
2006-08-25T18:56:13Z
2005
2005
http://hdl.handle.net/1721.1/33913
66911598
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005.
Includes bibliographical references (leaves 76-77).
The rise of prostate cancer diagnoses in the early 1990's due to better detection techniques and the recent advances in the clinical management of the disease have required better technologies for detection and monitoring disease status. The focus of my research at the Bioinstrumentation Laboratory was to design, fabricate and test an instrument for the preparation of excised frozen tissue from prostate cancer tumors in patients for conformal mapping of the sampling points to the local tissue geometry. This instrument was designed specifically for use on frozen samples that have been archived and the work involves solving issues inherent in extracting information from frozen tissue without denaturing DNA, RNA and proteins. Isolation of the nucleic acids from the bulk tissue and real time PCR amplification of a 152 bp segment of the androgen receptor was used to verify that the tissue was extracted successfully using the device. It is the intention of this work and others like it that quick and efficient mapping technologies will come into widespread use in predicting tumor size and growth rates thereby improving cancer patient diagnosis and treatment.
by Sasha R. Manoosingh.
S.M.
90 leaves
4480116 bytes
4483868 bytes
application/pdf
application/pdf
eng
Massachusetts Institute of Technology
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http://dspace.mit.edu/handle/1721.1/7582
Mechanical Engineering.
Tissue sampling array for tumor marker profiling
Thesis
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