2026-09-21T02:33:13Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/345782026-06-11T12:37:51Zcom_1721.1_7582com_1721.1_7581col_1721.1_131022
Gerald R. Fink.
Voynov, Vladimir
Massachusetts Institute of Technology. Dept. of Biology.
Massachusetts Institute of Technology. Department of Biology
2008-02-28T16:26:12Z
2008-02-28T16:26:12Z
2006
http://hdl.handle.net/1721.1/34578
71225146
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, June 2006.
"May 2006."
Includes bibliographical references.
A genome-wide screen for promoter-independent regulators of the Saccharomyces cerevisiae adhesin FLOII identified 37 novel regulators. Among the mutants with the strongest phenotype were the deletions of genes encoding subunits of the evolutionarily conserved THO complex. The THO complex, which is recruited to actively transcribed genes, is required for the efficient expression of FLO I and other yeast genes that have long internal tandem repeats. FLO I transcription elongation in Tho- mutants is hindered in the region of the tandem repeats, resulting in a loss of function. Moreover, the repeats become genetically unstable in Tho- mutants. A FLO I gene without the tandem repeats is transcribed equally well in Tho+ or Tho- strains. The Tho- defect in transcription is suppressed by overexpression of topoisomerase I, suggesting that the THO complex functions to rectify aberrant structures that arise during transcription.
by Vladimir Voynov.
Ph.D.
110 leaves
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
http://dspace.mit.edu/handle/1721.1/7582
Biology.
Genes with internal repeats require the THO complex for transcription
Thesis
application/pdf
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<Abstract>A genome-wide screen for promoter-independent regulators of the Saccharomyces cerevisiae adhesin FLOII identified 37 novel regulators. Among the mutants with the strongest phenotype were the deletions of genes encoding subunits of the evolutionarily conserved THO complex. The THO complex, which is recruited to actively transcribed genes, is required for the efficient expression of FLO I and other yeast genes that have long internal tandem repeats. FLO I transcription elongation in Tho- mutants is hindered in the region of the tandem repeats, resulting in a loss of function. Moreover, the repeats become genetically unstable in Tho- mutants. A FLO I gene without the tandem repeats is transcribed equally well in Tho+ or Tho- strains. The Tho- defect in transcription is suppressed by overexpression of topoisomerase I, suggesting that the THO complex functions to rectify aberrant structures that arise during transcription.</Abstract>
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