2026-09-21T02:57:32Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/350662022-01-13T07:54:33Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
Joseph M. Jacobson.
Hsu, Byron B.
Massachusetts Institute of Technology. Dept. of Materials Science and Engineering.
Massachusetts Institute of Technology. Department of Materials Science and Engineering
2006-12-18T20:01:36Z
2006-12-18T20:01:36Z
2006
2006
http://hdl.handle.net/1721.1/35066
71229963
Includes bibliographical references (p. 19).
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2006.
Introduction: In biological research today, there is great demand for synthesized biological compounds. The sequencing of the Human Genome has been completed, as well as that of many other organisms. Current work is now shifting towards the production of biological macromolecules. More specifically, this includes gene and genome synthesis from user-defined sequences. A series of A, T, G, and C nucleotides are specified in advance, and then constructed. Because of the error rates in perfectly synthesizing these specific DNA chains, it is more efficient to synthesize smaller oligonudeotide chains (oligos) and then allow them to self-assemble them into a larger oligos. These assembled chains are then brought together to form even longer chains, in a repeating process known as hierarchical assembly.
by Byron Hsu.
S.B.
19 p.
850255 bytes
848034 bytes
application/pdf
application/pdf
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
http://dspace.mit.edu/handle/1721.1/7582
Materials Science and Engineering.
Fabrication and control of microfluidic devices for on-chip synthesis
Thesis
application/pdf
false
Publication
unknown
unknown
<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="97450f88-7c9b-4448-831e-5d47321e5222">
<Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type>
<Language>eng</Language>
<Title>Fabrication and control of microfluidic devices for on-chip synthesis</Title>
<PublishedIn>
<Publication>
</Publication>
</PublishedIn>
<PublicationDate>2006</PublicationDate>
<Authors>
<Author>
<DisplayName>Hsu, Byron B.</DisplayName>
<Affiliation>
<OrgUnit>
</OrgUnit>
</Affiliation>
</Author>
</Authors>
<Editors>
</Editors>
<Publishers>
<Publisher>
<DisplayName>Massachusetts Institute of Technology</DisplayName>
<OrgUnit />
</Publisher>
</Publishers>
<License>http://dspace.mit.edu/handle/1721.1/7582</License>
<Keyword>Materials Science and Engineering.</Keyword>
<Abstract>Introduction: In biological research today, there is great demand for synthesized biological compounds. The sequencing of the Human Genome has been completed, as well as that of many other organisms. Current work is now shifting towards the production of biological macromolecules. More specifically, this includes gene and genome synthesis from user-defined sequences. A series of A, T, G, and C nucleotides are specified in advance, and then constructed. Because of the error rates in perfectly synthesizing these specific DNA chains, it is more efficient to synthesize smaller oligonudeotide chains (oligos) and then allow them to self-assemble them into a larger oligos. These assembled chains are then brought together to form even longer chains, in a repeating process known as hierarchical assembly.</Abstract>
<Access xmlns="http://purl.org/coar/access_right"
>
</Access>
</Publication>