2026-09-19T16:59:52Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/356902022-01-13T07:54:23Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Cho Kyun Rha.
Kshetry, Nina
Massachusetts Institute of Technology. Dept. of Civil and Environmental Engineering.
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
2007-01-10T19:19:09Z
2007-01-10T19:19:09Z
2006
2006
http://hdl.handle.net/1721.1/35690
71301456
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2006.
Includes bibliographical references (leaves 79-83).
Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHB within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using 13C NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHB inclusions is proposed.
by Nina Kshetry.
S.M.
83 leaves
22238044 bytes
22237606 bytes
application/pdf
application/pdf
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
http://dspace.mit.edu/handle/1721.1/7582
Civil and Environmental Engineering.
Isolation and characterization of bacterial polyhydroxybutyrate inclusions
Thesis
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