2026-09-19T18:44:43Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/362802022-01-13T07:54:21Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
Sarah E. O'Connor.
Wu, Katherine A. (Katherine Ann)
Massachusetts Institute of Technology. Dept. of Chemistry.
Massachusetts Institute of Technology. Department of Chemistry
2007-02-21T13:20:56Z
2007-02-21T13:20:56Z
2005
2005
http://hdl.handle.net/1721.1/36280
77529661
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2005.
Includes bibliographical references (leaves 20-21).
Inhibition of the chaperone protein Hsp90 in plants and insects has been found to result in drastic changes in phenotype. We investigated the effect of Hsp90 inhibition on the bacteria Streptomyces coelicolor. These changes were studied by growing S. coelicolor in the presence of Hsp90 inhibitors, extracting the growth media, and subjecting the extractions to HPLC and MS analysis. It has been found that reproducibility is difficult to achieve. Since the genome of S. coelicolor has been sequenced, attention was turned to studying changes in mRNA expression levels, which gave more reproducible results, as well as insight to the specific genes being affected.
by Katherine A. Wu.
S.B.
21 leaves
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
http://dspace.mit.edu/handle/1721.1/7582
Chemistry.
Inhibition of Hsp90 in Streptomyces coelicolor
Inhibition of Hsp90 in S. coelicolor
Thesis
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