2026-09-20T10:19:49Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/372002022-01-13T07:54:29Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Joseph M. Jacobson.
Emig, Christopher Joseph
Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
2007-04-20T15:48:40Z
2007-04-20T15:48:40Z
2006
2006
http://hdl.handle.net/1721.1/37200
78914934
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2006.
Includes bibliographical references (leaves 56-58).
Molecular and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled into large constructs of DNA for costs significantly less than traditional methods. This thesis presents a complete platform for the photoelectrochemical cleavage of protecting groups during in situ DNA synthesis. Photoelectrochemically patterned microarrays of phosphoramidite dye were produced with spot sizes of 100 um. This technology holds the potential for lowest cost per base pair of DNA produced over alternative microarray technologies, and may prove to be useful in de novo synthesis of large DNA constructs.
by Christopher Joseph Emig.
M.Eng.
62 leaves
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
http://dspace.mit.edu/handle/1721.1/7582
Electrical Engineering and Computer Science.
Photoelectrochemical array platform for genomic scale DNA synthesis
Thesis
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