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Ian Hunter. David, Robert, 1977- Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Department of Mechanical Engineering 2007-08-03T15:41:31Z 2007-08-03T15:41:31Z 2006 2006 http://hdl.handle.net/1721.1/38223 150982756 Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2006. Includes bibliographical references (p. 95-104). Labeling methods with optical readout are widely used to implement high throughput screens for drug discovery. However, labeling requires assay customization and does not allow examination of the reactants in their native state. The most direct and universal non-labeling method is calorimetry, but current calorimetric techniques are limited in resolution and throughput for pharmaceutical applications. In this thesis, a novel single-reaction microcalorimeter with optical readout, based on liquid expansion, was designed and built. The instrument was first constructed as a miniature liquid-in-glass thermometer in which the meniscus level was read by a Michelson interferometer. Contact angle hysteresis was limited by a wetting film and the low meniscus velocity. The sub-microKelvin resolution achieved was the lowest known for any thermometer above cryogenic temperatures. The thermometer was modified for use as a batch analysis microcalorimeter. Special attention was paid to minimize evaporation of the 1 /L reaction drops. Resolution of approximately 10 pJ was achieved for an acid dilution. by Robert David. Ph.D. 104 p. eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Mechanical Engineering. A liquid-in-glass thermometer with sub-microKelvin resolution, and its application for calorimetry Thesis application/pdf Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="4145469d-984c-4bf0-acd4-a6de5ee59d2f"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>A liquid-in-glass thermometer with sub-microKelvin resolution, and its application for calorimetry</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2006</PublicationDate> <Authors> <Author> <DisplayName>David, Robert, 1977-</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Mechanical Engineering.</Keyword> <Abstract>Labeling methods with optical readout are widely used to implement high throughput screens for drug discovery. However, labeling requires assay customization and does not allow examination of the reactants in their native state. The most direct and universal non-labeling method is calorimetry, but current calorimetric techniques are limited in resolution and throughput for pharmaceutical applications. In this thesis, a novel single-reaction microcalorimeter with optical readout, based on liquid expansion, was designed and built. The instrument was first constructed as a miniature liquid-in-glass thermometer in which the meniscus level was read by a Michelson interferometer. Contact angle hysteresis was limited by a wetting film and the low meniscus velocity. The sub-microKelvin resolution achieved was the lowest known for any thermometer above cryogenic temperatures. The thermometer was modified for use as a batch analysis microcalorimeter. Special attention was paid to minimize evaporation of the 1 /L reaction drops. Resolution of approximately 10 pJ was achieved for an acid dilution.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>