2026-09-24T00:08:59Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/404632022-01-13T07:54:36Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
Myron Spector.
Pfeiffer, Emily (Emily R.)
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Massachusetts Institute of Technology. Department of Mechanical Engineering
2008-02-27T22:28:18Z
2008-02-27T22:28:18Z
2007
2007
http://hdl.handle.net/1721.1/40463
191733957
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
Includes bibliographical references (p. 34-36).
This study examines glycosaminoglycan (GAG) density and aggregate compressive modulus HA of engineered cartilaginous implants. Culture parameters were developed to cause the goat articular chondrocyte seeded type II collagen scaffolds to generate 25 and 50% of the natural biochemical content of articular cartilage, with an overall goal of identifying construct compositions that might provide the most favorable response when implanted into defects in articular cartilage. Several scaffold cross-link densities were compared across constructs cultured in vitro to several time-points. The compressive modulus HA was measured through unconfined compression. One group of scaffolds averaged a compressive modulus one order of magnitude below that of natural tissue. Histological analysis verified that a chondrogenic phenotype was maintained and revealed a concentration of tissue development in the center of most scaffolds. This work includes a design for an original mechanical test apparatus for measuring the Poisson's ratio of the samples, enabling meaningful interpretation of indentation test results.
by Emily Pfeiffer.
S.B.
42 p.
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
http://dspace.mit.edu/handle/1721.1/7582
Mechanical Engineering.
The effects of glycosaminoglycan content on the compressive modulus of chondrocyte seeded type II collagen scaffolds
Effects of GAG content on the compressive chondrocyte seeded type II collagen scaffolds
Thesis
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<Abstract>This study examines glycosaminoglycan (GAG) density and aggregate compressive modulus HA of engineered cartilaginous implants. Culture parameters were developed to cause the goat articular chondrocyte seeded type II collagen scaffolds to generate 25 and 50% of the natural biochemical content of articular cartilage, with an overall goal of identifying construct compositions that might provide the most favorable response when implanted into defects in articular cartilage. Several scaffold cross-link densities were compared across constructs cultured in vitro to several time-points. The compressive modulus HA was measured through unconfined compression. One group of scaffolds averaged a compressive modulus one order of magnitude below that of natural tissue. Histological analysis verified that a chondrogenic phenotype was maintained and revealed a concentration of tissue development in the center of most scaffolds. This work includes a design for an original mechanical test apparatus for measuring the Poisson's ratio of the samples, enabling meaningful interpretation of indentation test results.</Abstract>
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