2026-09-18T22:51:26Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/422992022-01-13T07:54:36Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Peter T.C. So.
Cha, Jae Won
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Massachusetts Institute of Technology. Department of Mechanical Engineering
2008-09-03T15:16:14Z
2008-09-03T15:16:14Z
2007
2007
http://hdl.handle.net/1721.1/42299
232359016
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
Includes bibliographical references (leaves 45-46).
Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread function at the focal point becomes broadened resulting in degraded resolution and lower signal. With an adaptive optics system, which consists of a wavefront camera and deformable mirror, the wavefront distortion can be measured and corrected. By correcting the distorted wavefront with adaptive optics, resolution and signal level can be preserved at greater imaging depth.
by Jae Won Cha.
S.M.
49 leaves
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by
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http://dspace.mit.edu/handle/1721.1/7582
Mechanical Engineering.
Extending imaging depth of multiphoton microscopy
Thesis
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<Abstract>Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread function at the focal point becomes broadened resulting in degraded resolution and lower signal. With an adaptive optics system, which consists of a wavefront camera and deformable mirror, the wavefront distortion can be measured and corrected. By correcting the distorted wavefront with adaptive optics, resolution and signal level can be preserved at greater imaging depth.</Abstract>
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