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Angelika Amon. Rahal, Rami S Massachusetts Institute of Technology. Dept. of Biology. Massachusetts Institute of Technology. Department of Biology 2008-12-11T18:22:38Z 2008-12-11T18:22:38Z 2008 2008 http://hdl.handle.net/1721.1/43758 259777964 Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2008. Includes bibliographical references. Mitotic cyclin-dependent kinases (CDKs) are best known for their essential functions in triggering entry into M-phase, where they have established roles in nuclear envelope breakdown, chromosome condensation, and Golgi fragmentation. Whether mitotic CDKs have essential functions within mitosis is unclear. The work presented in this thesis indicates that mitotic CDKs, Clbl/2-CDKs or Clb2/3-CDKs, have two essential roles in mitotic progression. First, mitotic CDK activity was found to be required for the timely degradation of Securin/Pds1 at the metaphase-anaphase transition, demonstrating a requirement for mitotic CDKs in APC/C-Cdc20 activation in vivo. Second, mitotic CDK activity was necessary for the elongation of the mitotic spindle in anaphase. This requirement for mitotic CDKs is independent of cohesin removal and thus constitutes a novel role for CDK activity in mitotic progression. The dual roles of CDK in initiating loss of sister-chromatid cohesion and spindle elongation suggest a mechanism by which cells can couple and coordinate these events during mitosis. In addition, these results support a model in which different CDK activity threshold requirements help establish the order of events within in M phase, thereby ensuring cells initiate chromosome segregation only after they accumulate enough CDK activity to condense their chromosomes and to build a mitotic spindle. by Rami S. Rahal. Ph.D. 226, [467]-480, 79-90 p. eng Massachusetts Institute of Technology CDROM contains files in .mpg format. M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Biology. CDK control of mitotic progression in Saccharomyces cerevisiae Cyclin-dependent kinase control of mitotic progression in Saccharomyces cerevisiae Thesis application/pdf Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="23effa71-e7ef-454f-bc74-46fa219b251e"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>CDK control of mitotic progression in Saccharomyces cerevisiae</Title> <Subtitle>Cyclin-dependent kinase control of mitotic progression in Saccharomyces cerevisiae</Subtitle> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2008</PublicationDate> <Authors> <Author> <DisplayName>Rahal, Rami S</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Biology.</Keyword> <Abstract>Mitotic cyclin-dependent kinases (CDKs) are best known for their essential functions in triggering entry into M-phase, where they have established roles in nuclear envelope breakdown, chromosome condensation, and Golgi fragmentation. Whether mitotic CDKs have essential functions within mitosis is unclear. The work presented in this thesis indicates that mitotic CDKs, Clbl/2-CDKs or Clb2/3-CDKs, have two essential roles in mitotic progression. First, mitotic CDK activity was found to be required for the timely degradation of Securin/Pds1 at the metaphase-anaphase transition, demonstrating a requirement for mitotic CDKs in APC/C-Cdc20 activation in vivo. Second, mitotic CDK activity was necessary for the elongation of the mitotic spindle in anaphase. This requirement for mitotic CDKs is independent of cohesin removal and thus constitutes a novel role for CDK activity in mitotic progression. The dual roles of CDK in initiating loss of sister-chromatid cohesion and spindle elongation suggest a mechanism by which cells can couple and coordinate these events during mitosis. In addition, these results support a model in which different CDK activity threshold requirements help establish the order of events within in M phase, thereby ensuring cells initiate chromosome segregation only after they accumulate enough CDK activity to condense their chromosomes and to build a mitotic spindle.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>