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Ioannis V. Yannas. Wong, Matthew Q Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Department of Mechanical Engineering 2009-03-16T19:55:14Z 2009-03-16T19:55:14Z 2008 2008 http://hdl.handle.net/1721.1/44881 302286544 Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2008. Includes bibliographical references (leaves 49-53). This study examines the transcriptional differences between nerve wounds treated with silicone tubes and those treated with collagen nerve regeneration templates. The primary motivation for the study is to test the hypothesis that the discrepancy between the low-quality regenerate that forms in a silicone tube and the high-quality regenerate that forms in a collagen tube is due to differences in gene transcription. We used in vivo experiments on rat sciatic nerves with the use of real-time PCR to study the mRNA expression levels of certain inflammatory cytokines and contractile proteins after injury in both silicone-treated and collagen-treated nerve wounds. The contents of the wound site were analyzed on days 1, 3, 7, 14, and 21 following injury. The results of this experiment showed that although a difference in mRNA expression of [alpha]-SM actin is significantly higher at later time points in silicone-treated wounded nerves as compared to collagen-treated wounded nerves, there is little or no significant difference in the mRNA expression of TGF-[beta]1, TGF-[beta]2, and TGF-[beta]3 in wounds from the two devices. This suggests that the presence of a collagen regeneration nerve template does not direct the wound healing process at a transcriptional level. by Matthew Quin-men Wong. S.M. 79 leaves eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Mechanical Engineering. Transcriptional analysis of the healing response of wounded nerves treated with collagen and silicone tubes Thesis application/pdf Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="d850de12-e37d-457d-a82d-21ca59b426df"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Transcriptional analysis of the healing response of wounded nerves treated with collagen and silicone tubes</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2008</PublicationDate> <Authors> <Author> <DisplayName>Wong, Matthew Q</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Mechanical Engineering.</Keyword> <Abstract>This study examines the transcriptional differences between nerve wounds treated with silicone tubes and those treated with collagen nerve regeneration templates. The primary motivation for the study is to test the hypothesis that the discrepancy between the low-quality regenerate that forms in a silicone tube and the high-quality regenerate that forms in a collagen tube is due to differences in gene transcription. We used in vivo experiments on rat sciatic nerves with the use of real-time PCR to study the mRNA expression levels of certain inflammatory cytokines and contractile proteins after injury in both silicone-treated and collagen-treated nerve wounds. The contents of the wound site were analyzed on days 1, 3, 7, 14, and 21 following injury. The results of this experiment showed that although a difference in mRNA expression of [alpha]-SM actin is significantly higher at later time points in silicone-treated wounded nerves as compared to collagen-treated wounded nerves, there is little or no significant difference in the mRNA expression of TGF-[beta]1, TGF-[beta]2, and TGF-[beta]3 in wounds from the two devices. This suggests that the presence of a collagen regeneration nerve template does not direct the wound healing process at a transcriptional level.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>