2026-09-20T17:47:08Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/453092022-01-13T07:54:15Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Peter K. Sorger.
Lu, Kuojung Gordon
Massachusetts Institute of Technology. Dept. of Biology.
Massachusetts Institute of Technology. Department of Biology
2009-04-29T17:23:44Z
2009-04-29T17:23:44Z
2008
2008
http://hdl.handle.net/1721.1/45309
313410867
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Biology, 2008.
Includes bibliographical references (leaves 39-43).
Protein phosphatases regulate the phosphorylation state of intracellular signaling molecules in conjunction with protein kinases. In order to better understand the role of phosphatases in the ErbB signaling network, experimental studies were carried out to validate assays and phosphatase inhibitors for gathering dynamic, system-wide data of phosphatase activity. We verified the use of In-Cell Westerns and phospho-ErbB ELISAs for these studies, as well as the use of different cell lines and both general and specific phosphatase inhibitors in system-wide experiments. The phosphatase inhibitors we used perturbed cellular systems in complex ways that can help elucidate the regulation and activity of specific phosphatases in the ErbB signaling pathway in the future.
by Kuojung Gordon Lu.
S.M.
61 leaves
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by
copyright. They may be viewed from this source for any purpose, but
reproduction or distribution in any format is prohibited without written
permission. See provided URL for inquiries about permission.
http://dspace.mit.edu/handle/1721.1/7582
Biology.
Investigations into the role of protein phosphatases in the EGFR signaling network
Thesis
application/pdf
Publication
unknown
unknown
<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="c5c5deb2-a3f4-4813-8f6d-0973500fba69">
<Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type>
<Language>eng</Language>
<Title>Investigations into the role of protein phosphatases in the EGFR signaling network</Title>
<PublishedIn>
<Publication>
</Publication>
</PublishedIn>
<PublicationDate>2008</PublicationDate>
<Authors>
<Author>
<DisplayName>Lu, Kuojung Gordon</DisplayName>
<Affiliation>
<OrgUnit>
</OrgUnit>
</Affiliation>
</Author>
</Authors>
<Editors>
</Editors>
<Publishers>
<Publisher>
<DisplayName>Massachusetts Institute of Technology</DisplayName>
<OrgUnit />
</Publisher>
</Publishers>
<License>http://dspace.mit.edu/handle/1721.1/7582</License>
<Keyword>Biology.</Keyword>
<Abstract>Protein phosphatases regulate the phosphorylation state of intracellular signaling molecules in conjunction with protein kinases. In order to better understand the role of phosphatases in the ErbB signaling network, experimental studies were carried out to validate assays and phosphatase inhibitors for gathering dynamic, system-wide data of phosphatase activity. We verified the use of In-Cell Westerns and phospho-ErbB ELISAs for these studies, as well as the use of different cell lines and both general and specific phosphatase inhibitors in system-wide experiments. The phosphatase inhibitors we used perturbed cellular systems in complex ways that can help elucidate the regulation and activity of specific phosphatases in the ErbB signaling pathway in the future.</Abstract>
<Access xmlns="http://purl.org/coar/access_right"
>
</Access>
</Publication>