2026-09-21T01:06:25Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/478152022-01-13T07:54:29Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Jongyoon Han.
Liu, Vincent Hok
Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
2009-10-01T15:46:24Z
2009-10-01T15:46:24Z
2009
2009
http://hdl.handle.net/1721.1/47815
429466732
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2009.
Includes bibliographical references (leaves 60-61).
A new generation of integrated preconcentrator and immunoassay was developed. A novel, self-aligned method for patterning Nafion resin was developed and applied to create a preconcentrator. In a parallel effort, a surface-based immunoassay was developed and optimized for use with the preconcentrator. Experiments were carried out with R-phycoerythrin (R-PE) to characterize the performance of the integrated preconcentrator and immunoassay. The immunoassay originally has a detection limit of Ing/mL of R-PE in 1xPBS. When coupled to the preconcentrator, this limit has been improved to 0.Olng/mL, a roughly 100x improvement. Discussions of the differences between this and previous generations of concentrators are also presented in this thesis.
by Vincent Hok Liu.
S.M.
61 leaves
eng
Massachusetts Institute of Technology
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http://dspace.mit.edu/handle/1721.1/7582
Electrical Engineering and Computer Science.
Development of an integrated capillary valve-based preconcentrator and surface-based immunoassay
Thesis
application/pdf
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