2026-09-20T12:07:31Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/516022022-01-13T07:54:41Zcom_1721.1_7582com_1721.1_7581col_1721.1_131024
George B. Benedek.
Grannan, Benjamin L. (Benjamin Lucas)
Massachusetts Institute of Technology. Dept. of Physics.
Massachusetts Institute of Technology. Department of Physics
2010-02-09T16:50:01Z
2010-02-09T16:50:01Z
2009
2009
http://hdl.handle.net/1721.1/51602
495719792
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Physics, 2009.
Includes bibliographical references (p. 39-40).
This thesis investigates the role of the protein cochlin and its isoforms in DFNA9 autosomal dominant late onset senorineural loss and vestibular disorder by quantifying the concentration of cochlin in the inner fluid called perilymph. Through the use of affinity chromatography, high performance liquid chromatography, the Bradford assay, western blot analysis, and proteomics analysis by mass spectroscopy, I acquired data which suggests that the concentration of cochlin in the perilymph of bovine calf ears is at most on the order of 10- mg/ml. I also determined the total protein concentration of native bovine perilymph to be 2.1+0.2 mg/ml. Additionally, I discuss the theory of quasielastic light scattering along with its relevance to understanding the role of cochlin in DFNA9.
by Benjamin L. Grannan.
S.B.
40 p.
eng
Massachusetts Institute of Technology
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http://dspace.mit.edu/handle/1721.1/7582
Physics.
Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss
Thesis
application/pdf
Publication
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<Abstract>This thesis investigates the role of the protein cochlin and its isoforms in DFNA9 autosomal dominant late onset senorineural loss and vestibular disorder by quantifying the concentration of cochlin in the inner fluid called perilymph. Through the use of affinity chromatography, high performance liquid chromatography, the Bradford assay, western blot analysis, and proteomics analysis by mass spectroscopy, I acquired data which suggests that the concentration of cochlin in the perilymph of bovine calf ears is at most on the order of 10- mg/ml. I also determined the total protein concentration of native bovine perilymph to be 2.1+0.2 mg/ml. Additionally, I discuss the theory of quasielastic light scattering along with its relevance to understanding the role of cochlin in DFNA9.</Abstract>
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