2026-09-19T00:00:46Zhttps://dspace.mit.edu/server/oai/request

oai:dspace.mit.edu:1721.1/592342022-01-13T07:54:33Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023

Francesco Stellacci. Carney, Randy (Randy Patrick) Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. Massachusetts Institute of Technology. Department of Materials Science and Engineering 2010-10-12T18:50:39Z 2010-10-12T18:50:39Z 2010 2010 http://hdl.handle.net/1721.1/59234 666483709 Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2010. Includes bibliographical references (p. 42-43). Analytical Ultracentrifugation (AUC) is a powerful tool to obtain statistically relevant size and shape measurements for macromolecular systems. Metal nanoparticles coated by a ligand shell of thiolated molecules provide diverse functionality, from targeted cellular delivery to the formation of complex assemblies. Here I show that AUC can be used to determine particle size distribution, ligand shell density, shape, and hydrodynamic radius. It can also be used to probe complex mixtures of nanoparticle assemblies, from 2D dimers and chains, to 3D trimers, tetramers, and higher order assemblies, from a consideration of their hydrodynamic shape factor and its relation to the sedimentation coefficient. With AUC, the ease of sample preparation, ligand shell information, and dramatic increase in sample size are improvements compared with electron microscopy, and the ability to probe multiple, discrete absorbing wavelengths and globally analyze with interference information offers a measured improvement compared with dynamic light scattering (DLS). This work describes multiple calibrations and considerations as well as theoretical contributions concerning the application of AUC to nanoparticle systems by Randy Carney. S.M. 43 p. eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Materials Science and Engineering. Probing metal nanoparticles and assemblies with analytical ultracentrifugation Synthesis, characterization, and fractionation of cell penetrating gold nanoparticles Thesis application/pdf Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="c93fbb2d-f5ed-448b-9f0f-7ea5473aa759"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Probing metal nanoparticles and assemblies with analytical ultracentrifugation</Title> <Subtitle>Synthesis, characterization, and fractionation of cell penetrating gold nanoparticles</Subtitle> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2010</PublicationDate> <Authors> <Author> <DisplayName>Carney, Randy (Randy Patrick)</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Materials Science and Engineering.</Keyword> <Abstract>Analytical Ultracentrifugation (AUC) is a powerful tool to obtain statistically relevant size and shape measurements for macromolecular systems. Metal nanoparticles coated by a ligand shell of thiolated molecules provide diverse functionality, from targeted cellular delivery to the formation of complex assemblies. Here I show that AUC can be used to determine particle size distribution, ligand shell density, shape, and hydrodynamic radius. It can also be used to probe complex mixtures of nanoparticle assemblies, from 2D dimers and chains, to 3D trimers, tetramers, and higher order assemblies, from a consideration of their hydrodynamic shape factor and its relation to the sedimentation coefficient. With AUC, the ease of sample preparation, ligand shell information, and dramatic increase in sample size are improvements compared with electron microscopy, and the ability to probe multiple, discrete absorbing wavelengths and globally analyze with interference information offers a measured improvement compared with dynamic light scattering (DLS). This work describes multiple calibrations and considerations as well as theoretical contributions concerning the application of AUC to nanoparticle systems</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>