2026-09-21T02:37:57Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/601882022-01-13T07:54:29Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Mehmet F. Yanik.
Steinmeyer, Joseph D. (Joseph Daly)
Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science.
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
2010-12-06T17:35:32Z
2010-12-06T17:35:32Z
2010
2010
http://hdl.handle.net/1721.1/60188
681919517
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2010.
Vita.
Includes bibliographical references (p. 37-39).
Single-cell electroporation is a technique for transfecting individual cells in tissue culture at relatively high efficiencies, however it is both time-consuming and low-throughput and this limits the number of different labeling agents that can be effectively introduced into a region of tissue in reasonable periods of time. A novel system that will rapidly load, clean, and accurately position a glass micropipette electrode into tissue culture for single-cell electroporation is proposed. The system will significantly increase the number of different labeling agents that can be introduced into a single tissue culture per unit time. This in turn, will provide a means for improving the study of neural anatomy at cellular resolutions in both tissue culture and in vivo environments.
Supported by grants from the National Institutes of Health and by the MIT Dept. of Electrical Engineering and Computer Science
by Joseph D. Steinmeyer.
S.M.
40 p.
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by
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http://dspace.mit.edu/handle/1721.1/7582
Electrical Engineering and Computer Science.
Rapid single-cell electroporation for labeling organotypic cultures
Thesis
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<Abstract>Single-cell electroporation is a technique for transfecting individual cells in tissue culture at relatively high efficiencies, however it is both time-consuming and low-throughput and this limits the number of different labeling agents that can be effectively introduced into a region of tissue in reasonable periods of time. A novel system that will rapidly load, clean, and accurately position a glass micropipette electrode into tissue culture for single-cell electroporation is proposed. The system will significantly increase the number of different labeling agents that can be introduced into a single tissue culture per unit time. This in turn, will provide a means for improving the study of neural anatomy at cellular resolutions in both tissue culture and in vivo environments.</Abstract>
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