2026-09-21T09:43:58Zhttps://dspace.mit.edu/server/oai/requestoai:dspace.mit.edu:1721.1/677932022-01-13T07:54:36Zcom_1721.1_7582com_1721.1_7581col_1721.1_131023
Cullen R. Buie.
Braff, William Allan
Massachusetts Institute of Technology. Dept. of Mechanical Engineering.
Massachusetts Institute of Technology. Department of Mechanical Engineering
2011-12-19T18:52:05Z
2011-12-19T18:52:05Z
2011
2011
http://hdl.handle.net/1721.1/67793
767822365
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2011.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 103-111).
Insulator-based dielectrophoresis (iDEP) is a very promising technique for sorting microparticles based on their electrical properties. By using constrictions in a microchannel to generate large electric field gradients, the need for microfabricated electrode arrays is eliminated. In this work, three-dimensional insulator-based dielectrophoresis (3DiDEP) devices are fabricated using a novel micromachining technique. These devices are predicted and observed to exhibit higher trapping sensitivity than existing devices in the literature; this allows for efficient trapping of microparticles and bacteria at low applied fields with minimal thermal shock on the particles due to Joule heating effects. The dielectric properties of a range of bacteria, as well as polystyrene beads are examined. The impact of these results on the development of microbial fuel cells and portable low-cost clinical diagnostic tools is also discussed.
by William Allan Braff.
S.M.
111 p.
eng
Massachusetts Institute of Technology
M.I.T. theses are protected by
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http://dspace.mit.edu/handle/1721.1/7582
Mechanical Engineering.
Manipulation of bacteria using three dimensional insulator based dielectrophoresis
Manipulation of bacteria using 3 dimensional insulator based dielectrophoresis
Manipulation of bacteria using 3D insulator based dielectrophoresis
Manipulation of bacteria using 3DiDEP
Manipulation of bacteria using three dimensional iDEP
Thesis
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Publication
unknown
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<Abstract>Insulator-based dielectrophoresis (iDEP) is a very promising technique for sorting microparticles based on their electrical properties. By using constrictions in a microchannel to generate large electric field gradients, the need for microfabricated electrode arrays is eliminated. In this work, three-dimensional insulator-based dielectrophoresis (3DiDEP) devices are fabricated using a novel micromachining technique. These devices are predicted and observed to exhibit higher trapping sensitivity than existing devices in the literature; this allows for efficient trapping of microparticles and bacteria at low applied fields with minimal thermal shock on the particles due to Joule heating effects. The dielectric properties of a range of bacteria, as well as polystyrene beads are examined. The impact of these results on the development of microbial fuel cells and portable low-cost clinical diagnostic tools is also discussed.</Abstract>
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