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Ron Weiss and Domitilla Del Vecchio. Lin, Allen Massachusetts Institute of Technology. Dept. of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science 2013-02-14T15:35:43Z 2013-02-14T15:35:43Z 2011 2011 http://hdl.handle.net/1721.1/76989 825553696 Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011. Cataloged from PDF version of thesis. Includes bibliographical references (p. 141-151). A central concept in synthetic biology is the reuse of well-characterized modules. Modularity simplifies circuit design by allowing for the decomposition of systems into separate modules for individual construction. Complex regulatory networks can be assembled from a library of devices. However, current devices in synthetic biology may not actually be modular and may instead change behavior upon interconnections, a phenomenon called retroactivity. Addition of a new component to a system can change individual device dynamics within the system, potentially making timeconsuming iterative redesign necessary. Another need for systems construction is the ability to rapidly assemble constructs from part libraries in a combinatorial, highthroughput fashion. In this thesis, a multi-site assembly method that permits the rapid reshuffling of promoters and genes for yeast expression is established. Synthetic circuits in yeast to measure retroactivity and to act as an insulator that attenuates such effect are designed and modeled. by Allen Lin. M.Eng. 151 p. eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Electrical Engineering and Computer Science. Retroactivity, modularity, and insulation in synthetic biology circuits Modularity, Retroactivity, and insulation in synthetic biology circuits Thesis application/pdf Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="91418982-40d7-42de-a072-b9678c9fa068"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Retroactivity, modularity, and insulation in synthetic biology circuits</Title> <Subtitle>Modularity, Retroactivity, and insulation in synthetic biology circuits</Subtitle> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2011</PublicationDate> <Authors> <Author> <DisplayName>Lin, Allen</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Electrical Engineering and Computer Science.</Keyword> <Abstract>A central concept in synthetic biology is the reuse of well-characterized modules. Modularity simplifies circuit design by allowing for the decomposition of systems into separate modules for individual construction. Complex regulatory networks can be assembled from a library of devices. However, current devices in synthetic biology may not actually be modular and may instead change behavior upon interconnections, a phenomenon called retroactivity. Addition of a new component to a system can change individual device dynamics within the system, potentially making timeconsuming iterative redesign necessary. Another need for systems construction is the ability to rapidly assemble constructs from part libraries in a combinatorial, highthroughput fashion. In this thesis, a multi-site assembly method that permits the rapid reshuffling of promoters and genes for yeast expression is established. Synthetic circuits in yeast to measure retroactivity and to act as an insulator that attenuates such effect are designed and modeled.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>