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Mehmet Fatih Yanik. Chang, Tsung-Yao, Ph. D. Massachusetts Institute of Technology Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science 2013-04-12T19:24:24Z 2013-04-12T19:24:24Z 2012 2012 http://hdl.handle.net/1721.1/78443 831437348 Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2012. Cataloged from PDF version of thesis. Includes bibliographical references. High-throughput screening (HTS) is seen as one of the most promising technologies to facilitate biomedical studies and pharmaceutical discoveries. Although large varieties of in vitro HTS technologies have opened great opportunities, the speed of improvement has been limited by lack of advanced tools for in vivo screening on whole complex organisms, such as vertebrates. To address this issue, a high-throughput platform as a vertebrate total analysis/screening system (V-TAS) is proposed. This platform consists of two independent parts: an automated imaging system and an automated microinjection system. These two systems are designed for general high-content high-throughput pharmaceutical and genetic screens on whole zebrafish larvae, and therefore, are well-modularized for adapting different situations. Furthermore, to demonstrate the capability of V-TAS, a screen of lipidoid library for biologics delivery on thousands of animals was conducted. Very limited damage to the larvae was shown during the screening. In the end, the author also validated the hits discovered by V-TAS can be applied to more advanced animal models such as rats, and be more predictable than cell-based assays. by Tsung-Yao Chang. Ph.D. 76 p. eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Electrical Engineering and Computer Science. High-throughput vertebrate total analysis/screening platform Thesis application/pdf Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="bddb197c-e830-42af-88bf-b96d18f8f65e"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>High-throughput vertebrate total analysis/screening platform</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2012</PublicationDate> <Authors> <Author> <DisplayName>Chang, Tsung-Yao, Ph. D. Massachusetts Institute of Technology</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Electrical Engineering and Computer Science.</Keyword> <Abstract>High-throughput screening (HTS) is seen as one of the most promising technologies to facilitate biomedical studies and pharmaceutical discoveries. Although large varieties of in vitro HTS technologies have opened great opportunities, the speed of improvement has been limited by lack of advanced tools for in vivo screening on whole complex organisms, such as vertebrates. To address this issue, a high-throughput platform as a vertebrate total analysis/screening system (V-TAS) is proposed. This platform consists of two independent parts: an automated imaging system and an automated microinjection system. These two systems are designed for general high-content high-throughput pharmaceutical and genetic screens on whole zebrafish larvae, and therefore, are well-modularized for adapting different situations. Furthermore, to demonstrate the capability of V-TAS, a screen of lipidoid library for biologics delivery on thousands of animals was conducted. Very limited damage to the larvae was shown during the screening. In the end, the author also validated the hits discovered by V-TAS can be applied to more advanced animal models such as rats, and be more predictable than cell-based assays.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>