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Krystyn J. Van Vliet. Jolibois-Quinot, Remi Massachusetts Institute of Technology. Dept. of Materials Science and Engineering. Massachusetts Institute of Technology. Department of Materials Science and Engineering 2013-07-10T14:54:46Z 2013-07-10T14:54:46Z 2012 2013 http://hdl.handle.net/1721.1/79561 851456035 Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, February 2013. Cataloged from PDF version of thesis. Includes bibliographical references (p. 103-117). Mesenchymal stem cells (MSCs) are derived from bone marrow, and are capable of proliferating and differentiating along multiple pathways such as osteoblasts, chondrocytes and adipocytes. MSCs offer the means for regenerative therapies not possible with conventional small molecule/antibody/ nucleic acid therapeutics. However, all MSCs are not equivalent. Adult MSCs (aMSCs) derived from infant or adult sources are heterogeneous, exhibit poor overall integration in host tissues, and their differentiation and proliferation capacities are limited by ex vivo culture. On the other hand, fetal MSCs (fMSCs) derived from fetuses are more homogeneous, plastic and grow faster than aMSCs. However, they face serious ethical and practical issues that limit their applications. For these reasons, we hypothesized that aMSC populations contain a subpopulation with similar biophysical and biological properties to fMSCs. To verify this thesis, we studied aMSC size distribution, aMSC migration velocity and aMSC mechanical properties. We explain later in this work why we chose these characteristics. We were then able to find a subpopulation of aMSCs with similar size distribution to fMSCs. We were not able to find a subpopulation of aMSCs with similar migration velocity to fMSCs. At last, we were able to prove the existence of a subpopulation of aMSCs with similar mechanical properties to fMSCs. by Remi Jolibois-Quinot. S.M. 126 p. eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Materials Science and Engineering. Quantitative correlations among human mesenchymal stem cell mechanical properties and biological function Quantitative correlations among human MSC mechanical properties and biological function Thesis application/pdf false Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="afa9eef7-7f52-4f08-892e-9a00610bac19"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Quantitative correlations among human mesenchymal stem cell mechanical properties and biological function</Title> <Subtitle>Quantitative correlations among human MSC mechanical properties and biological function</Subtitle> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2013</PublicationDate> <Authors> <Author> <DisplayName>Jolibois-Quinot, Remi</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Materials Science and Engineering.</Keyword> <Abstract>Mesenchymal stem cells (MSCs) are derived from bone marrow, and are capable of proliferating and differentiating along multiple pathways such as osteoblasts, chondrocytes and adipocytes. MSCs offer the means for regenerative therapies not possible with conventional small molecule/antibody/ nucleic acid therapeutics. However, all MSCs are not equivalent. Adult MSCs (aMSCs) derived from infant or adult sources are heterogeneous, exhibit poor overall integration in host tissues, and their differentiation and proliferation capacities are limited by ex vivo culture. On the other hand, fetal MSCs (fMSCs) derived from fetuses are more homogeneous, plastic and grow faster than aMSCs. However, they face serious ethical and practical issues that limit their applications. For these reasons, we hypothesized that aMSC populations contain a subpopulation with similar biophysical and biological properties to fMSCs. To verify this thesis, we studied aMSC size distribution, aMSC migration velocity and aMSC mechanical properties. We explain later in this work why we chose these characteristics. We were then able to find a subpopulation of aMSCs with similar size distribution to fMSCs. We were not able to find a subpopulation of aMSCs with similar migration velocity to fMSCs. At last, we were able to prove the existence of a subpopulation of aMSCs with similar mechanical properties to fMSCs.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>