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Linda G. Griffith. Fujii, Jennifer T. (Jennifer Tomiko), 1972- Massachusetts Institute of Technology. Dept. of Chemical Engineering. Massachusetts Institute of Technology. Department of Chemical Engineering 2005-08-23T15:38:48Z 2005-08-23T15:38:48Z 2000 2000 http://hdl.handle.net/1721.1/8826 48385721 Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2000. Includes bibliographical references. Controlling the spatial distribution of cells in two and three dimensions may be important in the design of advanced tissue engineering scaffolds and other biomedical applications. In this thesis, the concept of biophysical sorting was applied as a method to control the spatial distribution of cells. This approach relies on a self-assembly process that is dependent, in part, on the intrinsic adhesivity of cells. A model system was developed using a simple patterning technique to prepare surfaces with alternating regions that supported variable cell response. First, the influence of certain biophysical parameters that may govern multicellular assembly of a single cell type on patterned surfaces was quantitatively investigated. For surfaces patterned with small features that allow cells to sample surrounding regions through membrane protrusions, it was found that a dynamic equilibrium distribution of cells correlated with differen~es in cell-substratum adhesion strength. The approach to that distribution, however, could be kinetically limited by the inability of the individual cells to sample adjacent areas of the patterned surface. This kinetic limitation was studied on surfaces with increasingly large feature sizes, and found that a simple diffusion model of migration may not completely describe the present system. Other effects such as contact inhibited motility and an induction time for migration may also influence multicellular assembly. The potential of multicellular assembly to simultaneously control the distribution of two cell types was also investigated. First, the multicellular assembly of each cell type was studied in isolation. Co-culture experiments indicated that, in addition to the factors that govern the assembly of a single cell type, sorting of two cell types depended on cell density. Images of high cell density co-cultures suggest that incomplete biophysical separation was achieved. by Jennifer T. Fujii. Ph.D. 173 leaves 12033234 bytes 12032988 bytes application/pdf application/pdf eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Chemical Engineering. Multicellular self-assembly on patterned surfaces Thesis application/pdf false Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="239bdb6c-1d41-40f1-94e2-438a70153eed"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Multicellular self-assembly on patterned surfaces</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2000</PublicationDate> <Authors> <Author> <DisplayName>Fujii, Jennifer T. (Jennifer Tomiko), 1972-</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Chemical Engineering.</Keyword> <Abstract>Controlling the spatial distribution of cells in two and three dimensions may be important in the design of advanced tissue engineering scaffolds and other biomedical applications. In this thesis, the concept of biophysical sorting was applied as a method to control the spatial distribution of cells. This approach relies on a self-assembly process that is dependent, in part, on the intrinsic adhesivity of cells. A model system was developed using a simple patterning technique to prepare surfaces with alternating regions that supported variable cell response. First, the influence of certain biophysical parameters that may govern multicellular assembly of a single cell type on patterned surfaces was quantitatively investigated. For surfaces patterned with small features that allow cells to sample surrounding regions through membrane protrusions, it was found that a dynamic equilibrium distribution of cells correlated with differen~es in cell-substratum adhesion strength. The approach to that distribution, however, could be kinetically limited by the inability of the individual cells to sample adjacent areas of the patterned surface. This kinetic limitation was studied on surfaces with increasingly large feature sizes, and found that a simple diffusion model of migration may not completely describe the present system. Other effects such as contact inhibited motility and an induction time for migration may also influence multicellular assembly. The potential of multicellular assembly to simultaneously control the distribution of two cell types was also investigated. First, the multicellular assembly of each cell type was studied in isolation. Co-culture experiments indicated that, in addition to the factors that govern the assembly of a single cell type, sorting of two cell types depended on cell density. Images of high cell density co-cultures suggest that incomplete biophysical separation was achieved.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>