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Catherine L. Drennan. Funk, Michael A. (Michael Andrew) Massachusetts Institute of Technology. Department of Chemistry. Massachusetts Institute of Technology. Department of Chemistry 2015-09-17T19:14:08Z 2015-09-17T19:14:08Z 2015 2015 http://hdl.handle.net/1721.1/98816 921148237 Thesis: Ph. D. in Biological Chemistry, Massachusetts Institute of Technology, Department of Chemistry, 2015. Vita. Cataloged from PDF version of thesis. Includes bibliographical references. Anaerobic bacteria play a crucial role in cycling of nutrients in diverse ecosystems, degradation of organic compounds, and as key members of the human gut microbiome. The absence of oxygen limits the chemistry that bacteria can perform; however, these organisms do make use of organic radical cofactors that are oxygen sensitive. This thesis presents a structural analysis of three enzymes that utilize a glycyl radical cofactor to perform difficult, radical-based chemistry. Benzylsuccinate synthase catalyzes the first step in the anaerobic degradation of toluene, a major component of gasoline and an environmental pollutant. Choline trimethylamine-lyase is used by gut bacteria to degrade choline, producing a byproduct, trimethylamine, which is linked to human diseases. These two enzymes share a common protein fold and also utilize a similar series of steps to initiate chemistry on their substrates. However, once they have generated a radical intermediate, the enzyme active site guides very different chemical steps in these two enzymes. Class III ribonucleotide reductases catalyze the same chemical reaction as observed in aerobic or oxygen independent systems, but utilize a glycyl radical cofactor to initiate chemistry. X-ray crystal structures have given us snapshots of these enzymes in action and accompanying biochemical experiments are help reveal the mechanisms they use to control radical chemistry. These structures have highlighted the potential diversity of chemical reactions available to anaerobic organisms through the use of a conserved enzyme fold. by Michael A. Funk. Ph.D. in Biological Chemistry 234 pages eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Chemistry. Structural studies of radical enzymes in bacterial central metabolism Thesis application/pdf false Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="4c872ceb-86e3-43bd-b1f9-aee0a9b759cd"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Structural studies of radical enzymes in bacterial central metabolism</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2015</PublicationDate> <Authors> <Author> <DisplayName>Funk, Michael A. (Michael Andrew)</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Chemistry.</Keyword> <Abstract>Anaerobic bacteria play a crucial role in cycling of nutrients in diverse ecosystems, degradation of organic compounds, and as key members of the human gut microbiome. The absence of oxygen limits the chemistry that bacteria can perform; however, these organisms do make use of organic radical cofactors that are oxygen sensitive. This thesis presents a structural analysis of three enzymes that utilize a glycyl radical cofactor to perform difficult, radical-based chemistry. Benzylsuccinate synthase catalyzes the first step in the anaerobic degradation of toluene, a major component of gasoline and an environmental pollutant. Choline trimethylamine-lyase is used by gut bacteria to degrade choline, producing a byproduct, trimethylamine, which is linked to human diseases. These two enzymes share a common protein fold and also utilize a similar series of steps to initiate chemistry on their substrates. However, once they have generated a radical intermediate, the enzyme active site guides very different chemical steps in these two enzymes. Class III ribonucleotide reductases catalyze the same chemical reaction as observed in aerobic or oxygen independent systems, but utilize a glycyl radical cofactor to initiate chemistry. X-ray crystal structures have given us snapshots of these enzymes in action and accompanying biochemical experiments are help reveal the mechanisms they use to control radical chemistry. These structures have highlighted the potential diversity of chemical reactions available to anaerobic organisms through the use of a conserved enzyme fold.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>