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Neri Oxman. Patrick, William Graham, S.M. Massachusetts Institute of Technology Massachusetts Institute of Technology. Department of Materials Science and Engineering. Massachusetts Institute of Technology. Department of Materials Science and Engineering 2015-09-29T18:08:45Z 2015-09-29T18:08:45Z 2015 2015 http://hdl.handle.net/1721.1/98918 921147345 Thesis: S.M., Massachusetts Institute of Technology, Department of Materials Science and Engineering, 2015. This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections. Cataloged from student-submitted PDF version of thesis. Includes bibliographical references (pages 111-120). Synthetic biology is a rapidly growing engineering discipline widely used in biotechnological applications. However, there are few examples of using synthetic biology in product design and there are even fewer - perhaps no - examples of incorporating fluids containing synthetic organisms and biomolecules into a product. The goals of this thesis are two-fold. First, the author investigates how to contain and control fluids in 3D printed fluid channels. 3D printing methods are characterized by their ability to create fluidic channels that are compatible with biochemistry and culturing microorganisms. Second, the author explores how to design the materiality and geometry of the fluid channels to affect biological function. These goals are pursued in two distinct projects: DNA assembly in 3D printed fluidics and Mushtari, a fluidic wearable designed to contain cyanobacteria and E. coli cultures. Contributions include (1) characterizing the resolution of three 3D printing methods for creating fluidic channels, (2) demonstrating compatibility of 3D printing methods with cell culture and DNA assembly biochemistry, (3) demonstrating the capability to print wearable-scale millifluidic networks up to 58 meters in length, and (4) developing approaches for fabricating geometrically complex fluidic systems. by William Graham Patrick. S.M. 120 pages eng Massachusetts Institute of Technology M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 Materials Science and Engineering. Growing a second skin : towards synthetic biology in product design Thesis application/pdf false Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="30fecfe5-04b2-46e7-afdc-21842df44aac"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Growing a second skin : towards synthetic biology in product design</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2015</PublicationDate> <Authors> <Author> <DisplayName>Patrick, William Graham, S.M. Massachusetts Institute of Technology</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Materials Science and Engineering.</Keyword> <Abstract>Synthetic biology is a rapidly growing engineering discipline widely used in biotechnological applications. However, there are few examples of using synthetic biology in product design and there are even fewer - perhaps no - examples of incorporating fluids containing synthetic organisms and biomolecules into a product. The goals of this thesis are two-fold. First, the author investigates how to contain and control fluids in 3D printed fluid channels. 3D printing methods are characterized by their ability to create fluidic channels that are compatible with biochemistry and culturing microorganisms. Second, the author explores how to design the materiality and geometry of the fluid channels to affect biological function. These goals are pursued in two distinct projects: DNA assembly in 3D printed fluidics and Mushtari, a fluidic wearable designed to contain cyanobacteria and E. coli cultures. Contributions include (1) characterizing the resolution of three 3D printing methods for creating fluidic channels, (2) demonstrating compatibility of 3D printing methods with cell culture and DNA assembly biochemistry, (3) demonstrating the capability to print wearable-scale millifluidic networks up to 58 meters in length, and (4) developing approaches for fabricating geometrically complex fluidic systems.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>