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Jacquin C. Niles. Wall, Bridget (Bridget Anne) Massachusetts Institute of Technology. Department of Biological Engineering. Massachusetts Institute of Technology. Department of Biological Engineering 2015-09-29T18:09:03Z 2015-09-29T18:09:03Z 2015 2015 http://hdl.handle.net/1721.1/98923 921845784 Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2015. This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections. Cataloged from PDF student-submitted version of thesis. Includes bibliographical references (pages 120-136). New techniques to both prevent and treat the disease malaria are necessary. To develop these novel strategies, innovative tools must be designed to study the basic biology within Plasmodium falciparum and characteristics of the pathological relationship between host and parasite. These tools will be diverse in nature, yet all seek to address the same fundamental question: what are the characteristics of the parasite that can be exploited to decrease the burden this parasite places on the human species? First, the relationship between nitric oxide and the parasite-infected red blood cell will be measured using a microfluidic device. Second, a toolkit to determine the essentiality of genes of unknown function will be engineered and tested with three separate genes to improve and demonstrate usability. Third, a mutator strain will be engineered and defined for eventual use in the study of drug resistance and the characterization of the resistance potential of anti-malarial drugs. by Bridget Wall. Ph.D. 136 pages eng Massachusetts Institute of Technology MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 Biological Engineering. Engineered tools for studying the malaria parasite plasmodium falciparum Thesis application/pdf false Publication unknown unknown <Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="8b26e0c0-862f-4148-9b1f-5d1cd6d5b65b"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Engineered tools for studying the malaria parasite plasmodium falciparum</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2015</PublicationDate> <Authors> <Author> <DisplayName>Wall, Bridget (Bridget Anne)</DisplayName> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Massachusetts Institute of Technology</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://dspace.mit.edu/handle/1721.1/7582</License> <Keyword>Biological Engineering.</Keyword> <Abstract>New techniques to both prevent and treat the disease malaria are necessary. To develop these novel strategies, innovative tools must be designed to study the basic biology within Plasmodium falciparum and characteristics of the pathological relationship between host and parasite. These tools will be diverse in nature, yet all seek to address the same fundamental question: what are the characteristics of the parasite that can be exploited to decrease the burden this parasite places on the human species? First, the relationship between nitric oxide and the parasite-infected red blood cell will be measured using a microfluidic device. Second, a toolkit to determine the essentiality of genes of unknown function will be engineered and tested with three separate genes to improve and demonstrate usability. Third, a mutator strain will be engineered and defined for eventual use in the study of drug resistance and the characterization of the resistance potential of anti-malarial drugs.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication>