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Biological detection by means of mass reduction in a suspended microchannel resonator

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dc.contributor.advisor Scott R. Manalis. en_US Levy-Tzedek, Shelly en_US
dc.contributor.other Massachusetts Institute of Technology. Biological Engineering Division. en_US 2005-09-27T17:22:23Z 2005-09-27T17:22:23Z 2004 en_US 2004 en_US
dc.description Thesis (S.M.)--Massachusetts Institute of Technology, Biological Engineering Division, 2004. en_US
dc.description Includes bibliographical references (p. 33-37). en_US
dc.description.abstract Label-free detection is the detection of biomolecules and their interactions, without the use of a molecule external to the interaction, used as a reporter to indicate presence and/or location. The suspended microchannel resonator offers the opportunity to perform such label-free measurements. The goal of this work is to open new avenues of possible applications for the suspended channel. I introduce the concept of detecting mass subtraction as a new approach, rather than the conventional detection of mass addition. In a model implementation scenario of this approach, a mass-intensifying tag bound to a small ligand molecule will be equilibrated with surface-immobilized receptors, and later displaced by an identical, but label-free, ligand molecule. This approach offers opportunities to extend the sensitivity range of the device, as well as introduces new functionality for it. It enables researchers to follow, label-free, real-time enzymatic reactions, relative affinities of different ligands to a receptor, and presence of small molecules in a solution. en_US
dc.description.statementofresponsibility by Shelly Levy-Tzedek. en_US
dc.format.extent 37 p. en_US
dc.format.extent 2843473 bytes
dc.format.extent 2845328 bytes
dc.format.mimetype application/pdf
dc.format.mimetype application/pdf
dc.language.iso en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.subject Biological Engineering Division. en_US
dc.title Biological detection by means of mass reduction in a suspended microchannel resonator en_US
dc.type Thesis en_US S.M. en_US
dc.contributor.department Massachusetts Institute of Technology. Biological Engineering Division. en_US
dc.identifier.oclc 57569643 en_US

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