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  • Intracellular sensor spatial multiplexing via RNA scaffolds 

    Johnson, Shannon L. (Massachusetts Institute of Technology, 2019)
    To circumvent the limitations of spectrally multiplexing sensors, fluorescent sensors are clustered by type and spatially separated in the cytoplasm to avoid cross-talk. Each sensor is fused to an orthogonal viral capsid ...
  • Conducting polymers for electrochemically mediated separations 

    Ren, Yinying. (Massachusetts Institute of Technology, 2019)
    Many conventional separation technologies are limited by high energy costs, generation of chemical wastes, and lack of molecular selectivity. This thesis is motivated to develop novel separation techniques that overcome ...
  • Mechanistic insight on a chimeric Cas9 protein's specificity for DNA target with 5 '-NAA-3' PAM 

    Nip, Lisa. (Massachusetts Institute of Technology, 2019)
    Numerous protein variants have been made to expand the repertoire of CRISPR-Cas nucleases that can recognize protospacer-adjacent motifs (PAMs) other than the canonical NGG discovered in wild-type Streptococcus pyogenes. ...

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