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Glycocalyx acting as a mechanotransducer of fluid shear stress

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dc.contributor.advisor C. Forbes Dewey, Jr. en_US Yao, Yu, Ph. D. Massachusetts Institute of Technology en_US
dc.contributor.other Massachusetts Institute of Technology. Dept. of Mechanical Engineering. en_US 2007-01-10T16:56:47Z 2007-01-10T16:56:47Z 2005 en_US 2005 en_US
dc.description Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005. en_US
dc.description Includes bibliographical references (p. 51-56). en_US
dc.description.abstract It is widely recognized that fluid shearing forces acting on endothelial cells (ECs) have a profound effect on EC morphology, structure and function. Recent investigations in vivo have indicated the presence of a thick endothelial surface layer, also called the glycocalyx, with an estimated thickness on the scale of 0.5 pm, which restricts the flow of plasma and can exclude red blood cells and some macromolecular solutes. Based on our current understanding of signal transduction, we expect that the glycocalyx plays an essential role as a possible mechanotransducer of fluid shear stress to the actin cytoskeleton of the endothelial cells. We examined whether removing the glycocalyx can affect shear-induced cellular response, e.g. cell migration speed and wound edge healing. More specifically, we compared the two major components of the glycosaminoglycans in the glycocalyx, which are heparan sulfate GAGs and chondroitin sulafte GAGs. Our results showed that the removal of heparan sulfate GAGs has a much greater impact on the shear induced response compared to chondroitin sulfate GAGs, and the short term crawling speed response is highly suppressed in the first case. Therefore, it is highly possible that heparan sulfate GAGs is involved in the shear-induced signaling pathway. en_US
dc.description.statementofresponsibility by Yu Yao. en_US
dc.format.extent 56 p. en_US
dc.format.extent 2387305 bytes
dc.format.extent 2389595 bytes
dc.format.mimetype application/pdf
dc.format.mimetype application/pdf
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.subject Mechanical Engineering. en_US
dc.title Glycocalyx acting as a mechanotransducer of fluid shear stress en_US
dc.type Thesis en_US S.M. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Mechanical Engineering. en_US
dc.identifier.oclc 76761955 en_US

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