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Tissue-specific classification of alternatively spliced human exons

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dc.contributor.advisor Christopher Burge. en_US Rothman, Craig Jeremy en_US
dc.contributor.other Massachusetts Institute of Technology. Biological Engineering Division. en_US 2008-01-10T16:01:20Z 2008-01-10T16:01:20Z 2007 en_US 2007 en_US
dc.description Thesis (M. Eng.)--Massachusetts Institute of Technology, Biological Engineering Division, 2007. en_US
dc.description Includes bibliographical references (p. 53-57). en_US
dc.description.abstract Alternative splicing is involved in numerous cellular functions and is often disrupted and involved in disease. Previous research has identified methods to distinguish alternative conserved exons (ACEs) in human and mouse. However, the cellular machinery, the spliceosome, does not use comparative genomics to decide when to include and when to exclude an exon. Human RefSeq exons obtained from the University of California Santa Cruz (UCSC) genome browser were analyzed for tissue-specific skipping. Expressed sequence tags (ESTs) were aligned to exons and their tissue of origin and histology were identified. ACEs were also identified as a subset of the skipped exons. About 18% of the exons were identified as tissue-specifically skipped in one of sixteen different tissues at four stringency levels. The different datasets were analyzed for both general features such as exon and intron length, splice site strength, base composition, conservation, modularity, and susceptibility to nonsense-mediated mRNA decay caused by skipping. Cis-element motifs that might bind protein factors that affect splicing were identified using overrepresentation analysis and conserved occurrence rate between human and mouse. en_US
dc.description.abstract (cont.) Tissue-specific skipped exons were then classified with both a decision-tree based classifier (Random ForestsTM) and a support vector machine. Classification results were better for tissue-specific skipped exons vs. constitutive exons than for tissue-specific skipped exons vs. exons skipped in other tissues. en_US
dc.description.statementofresponsibility by Craig Jeremy Rothman. en_US
dc.format.extent 57 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. en_US
dc.subject Biological Engineering Division. en_US
dc.title Tissue-specific classification of alternatively spliced human exons en_US
dc.type Thesis en_US M.Eng. en_US
dc.contributor.department Massachusetts Institute of Technology. Biological Engineering Division. en_US
dc.identifier.oclc 182622079 en_US

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