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Requests for Comments (RFCs)

Research and Teaching Output of the MIT Community

Requests for Comments (RFCs)

 

The BioBricks Foundation is dedicated to promoting and protecting the open development, sharing, and reuse of BioBrick[TM] standard biological parts. Taking inspiration from the Internet Engineering Task Force, the BioBricks Foundation has implemented a Request for Comments process. A BioBricks Foundation Request for Comments, abbreviated RFC, is a short document that is intended for review by the rest of the synthetic biology community.

A BBF RFC might

  • propose a standard
  • describe best practices or protocols
  • provide information
  • comment, extend, or replace an earlier RFC

BBF RFC's are static documents or digital objects like videos intended to get an idea, proposed standard, or method out to the rest of the community for comment. BBF RFC's are numbered, for ease of referencing, and the numbers are assigned by the BioBricks Foundation.

Recent Submissions

  • Synthetic Biology Open Language Visual (SBOL Visual), version 1.0.0 
    Quinn, Jacqueline; Beal, Jacob; Bhatia, Swapnil; Cai, Patrick; Chen, Joanna; Clancy, Kevin; Hillson, Nathan; Galdzicki, Michal; Maheshwari, Akshay; P, Umesh; Pocock, Matthew; Rodriguez, Cesar; Stan, Guy-Bart; Endy, Drew (2013-03-31)
    In this BioBricks Foundation Request for Comments (BBF RFC), we specify the Synthetic Biology Open Language Visual standard (SBOL Visual) to enable consistent, human-readable depiction of genetic designs.
  • The GoldenBricks assembly: A standardized one-shot cloning technique for complete cassette assembly 
    Pauthenier, Cyrille; El-Sayyed, Hafez; Rostain, William; Cerisy, Tristan; Lopez, Carolina Gallo; Ferreira, Raphael; Moreira, Jorgelindo Da Veiga; Souterre, Tiffany; Eeckhout, Joachim; Nogue, Pierre Yves; Llamosi, Artemis; Parutto, Pierre; Nikolayeva, Iryna; Machat, Mohamed; Marquet, Clement; Młynarczyk, Anna; Thelie, Aurore; Landrain, Thomas; Tolonen, Andrew; Pollet, Nicolas; Faulon, Jean-Loup; Jaramilo, Alfonso (2012-11-05)
    BBF RFC 92 proposes a new standard assembly method for the Parts Registry. The method makes one-shot cloning of a complete eukaryotic or prokaryotic cassette possible in one day while keeping compatibility with the BBF RFC ...
  • Yeast Golden Gate: Standardized Assembly of S. cerevisiae Transcriptional Units 
    Boeke, Jef; Mitchell, Leslie; Cai, Yizhi; Stracquadanio, Giovanni; Chuang, James; Tan, Scott; Heston, Margo; Wang, Jiarui; Kim, Dong Won; Noronha, Anne Marie (2012-10-11)
    BBF RFC 88 describes a new standard for the assembly of basic Saccharomyces cerevisiae transcriptional units (TUs) consisting of a promoter/5’untranslated region (UTR), open reading frame (ORF), and 3’UTR/terminator. Note ...
  • The Measurement of rho‐independent Transcription Terminator Efficiency 
    He, Jiankui; Jing, Shui; Zhang, Mengshi; Liu, Xianggeng; Chen, Yao; Qie, Boyu; Shen, Liuxing; Wu, Zishan (2012-10-11)
    The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of terminators in bacteria. Because we have characterized the performance of terminator in E. coli and used a simple ...
  • The minimum information for a qualified BioBrick 
    Zhou, Mubing; Zhang, Junqiu; He, Jiankui (2012-10-11)
    Since the information of many existing BioBricks is incomplete, thus the usage of the BioBricks will be affected. It is necessary to standardize the minimum information required for a qualified BioBrick. Furthermore this ...
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