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Requests for Comments (RFCs)

Research and Teaching Output of the MIT Community

Requests for Comments (RFCs)


The BioBricks Foundation is dedicated to promoting and protecting the open development, sharing, and reuse of BioBrick[TM] standard biological parts. Taking inspiration from the Internet Engineering Task Force, the BioBricks Foundation has implemented a Request for Comments process. A BioBricks Foundation Request for Comments, abbreviated RFC, is a short document that is intended for review by the rest of the synthetic biology community.

A BBF RFC might

  • propose a standard
  • describe best practices or protocols
  • provide information
  • comment, extend, or replace an earlier RFC

BBF RFC's are static documents or digital objects like videos intended to get an idea, proposed standard, or method out to the rest of the community for comment. BBF RFC's are numbered, for ease of referencing, and the numbers are assigned by the BioBricks Foundation.

Recent Submissions

  • Ahlmann-Eltze, Constantin; Bunne, Charlotte; Büscher, Magdalena; Gleixner, Jan; Horn, Max; Huhn, Anna; Klughammer, Nils; Kreft, Jakob; Schäfer, Elisabeth; Schmelas, Carolin; Schmitz, Silvan; Waldhauer, Max; Bayer, Philipp; Krämer, Stephen; Neugebauer, Julia; Wehler, Pierre; Beaudouin, Joel; Di Ventura, Barbara; Eils, Roland (2015-03-17)
    This Request for Comments (RFC) proposes a new standard that allows for easy and flexible cloning of intein constructs and thus makes this technology accessible to the synthetic biology community.
  • Trubitsyna, Maryia; Chan, Karen; Cai, Yizhi; Elfick, Alistair; French, Chris (2015-03-17)
    This BioBrick Foundation Request for Comments (BBF RFC 104) describes a new approach to multiple part DNA assembly – BrickClip, which does not require use of any restriction enzymes, nor cloning of the parts to specific ...
  • Rutten, Virginia; Munabi, Angelina; Riche, Fergus; Lewy, Guy; Wilson, Hugh; Pipan, Miha; Bhate, Salil; Nghiem, Trang-Anh; Kaufhold, Will; Haseloff, Jim; Rubert, Alba; González, Alejandra; Quijano, Alfredo; Llopis, Ivan; Gavaldá, Jose; Estellés, Lucía; Vásquez, Marta; Orzáez, Diego; Deal, Cara; Gray, Jessica; Spiegel, Mischa; Monsey, Steven; Middlemiss, Alistair; Day, Jack; Patron, Nicola (2015-03-17)
    Here we define a standard syntax for assembling standard parts for expression in plant cells, extensible to all other eukaryotes. Variations of the Type IIS mediated cloning method known as Golden Gate Cloning, most notably ...
  • Wang, Lingjue; Tong, Yin; Zhou, Liqun; Ni, Zhuangdian; Zhu, Xiaodan; Li, Hanrun (2013-11-01)
    This Request for Comments (RFC) describes a new framework for standardize logic gene relations among gene circuits. Each type of logic module in gene circuit can be summarized in a standard device in electronics. In this ...
  • Wang, Lingjue; Zhu, Xiaodan; Li, Hanrun; He, Huiyu; Ni, Zhuangdian; Zhou, Liqun (2013-10-29)
    Current, there is well adopted documentation of biobricks. The Registry of Standard Biology Part set the standard documentation for biobrick. However, there is no standard way to document a genetic circuit, what information ...