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Genetic analysis of innate immunity in Caenorhabditis elegans

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dc.contributor.advisor Dennis Kim. en_US Redding, Bethany Joy en_US
dc.contributor.other Massachusetts Institute of Technology. Dept. of Biology. en_US 2008-12-11T18:32:41Z 2008-12-11T18:32:41Z 2007 en_US 2007 en_US
dc.description Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Biology, 2007. en_US
dc.description Includes bibliographical references (leaf 10). en_US
dc.description.abstract Pathogen resistance in Caenorhabditis elegans is a model for studying innate immunity. Several conserved activators in the p38 PMK-1 pathway have been identified and are required for pathogen resistance to the bacterium Pseudomonas aeroginosa. Thus far, screens have been performed to identify mutants that are susceptible to pathogen infection. Here, I will discuss a mek-1 suppressor screen that was performed to look for more components of the p38 PMK-1 pathway. Aided by the agls219 reporter, mutants with high levels of GFP expression were isolated and screened for suppression of the Esp phenotype of mek-1. Several independently isolated strong suppressor mutants were isolated and separated into possible complementation groups using pooled lysate SNP mapping. Interested to see if the mutants would also affect other stress response pathways in the worm, I tested the mutants for heavy metal sensitivity. Five out of the six strong Esp suppressor mutants did not suppress the heavy metal sensitivity of mek-1, suggesting that our mutants are more specifically affecting the pathogen resistance pathway and not the heavy metal stress response pathway. Future work will involve measuring PMK-1 phosphorylation levels using Western blots and fine mapping the mutants. en_US
dc.description.statementofresponsibility by Bethany Joy Redding. en_US
dc.format.extent 32 leaves en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.rights.uri en_US
dc.subject Biology. en_US
dc.title Genetic analysis of innate immunity in Caenorhabditis elegans en_US
dc.type Thesis en_US S.M. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Biology. en_US
dc.identifier.oclc 262616358 en_US

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