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Ecology and population structure of vibrionaceae in the coastal ocean

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dc.contributor.advisor Martin F. Polz. en_US Preheim, Sarah Pacocha en_US
dc.contributor.other Woods Hole Oceanographic Institution. en_US 2010-09-02T14:56:01Z 2010-09-02T14:56:01Z 2010 en_US 2010 en_US
dc.description Thesis (Ph.D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering; and the Woods Hole Oceanographic Institution), 2010. en_US
dc.description Cataloged from PDF version of thesis. en_US
dc.description Includes bibliographical references. en_US
dc.description.abstract Extensive genetic diversity has been discovered in the microbial world, yet mechanisms that shape and maintain this diversity remain poorly understood. This thesis investigates to what extent populations of the gamma-proteobacterial family, Vibrionaceae, are ecologically specialized by investigating the distribution across a wide range of environmental categories, such as marine invertebrates or particles in the water column. Additionally, it seeks to determine whether in situ population distributions directly result from a competitive advantage over other Vibrio populations. This was investigated by in vitro competition assays on mixtures of native, sterilized particles. Generalist populations were found to dominate the associations with marine invertebrates, consistent with a model of high migration dominated population assembly. A majority of populations occurred broadly within and among the different types of invertebrates sampled, with one population being a near perfect generalist with regard to seasons, host taxa and body regions. High variability across host individuals, consistent with a scenario of stochastic clonal expansion, was especially pronounced in crab and zooplankton samples. Specialization, demonstrated by specific and reproducible association with different particle types in the water column, is more common than specialization within invertebrate hosts. en_US
dc.description.abstract (cont.) Co-existing Vibrio species show strong preferences for different types of particulate matter in the water column suggesting that competition for limited resources influences their evolution. While populations show different growth profiles on particle derived substrates, relative growth advantages of specialist populations in competition with other Vibrio populations on native particles may not be sufficient to explain observed environmental distributions. Instead, populations may gain an advantage on these particles by colonizing the living plant or zooplankton prior to death and degradation into particulate matter. In summary, although vibrios are known commensals of marine invertebrates, evidence suggests that population structure within animals is fairly weak compared to suspended particles in the water column. This highlights the importance of comparing multiple environmental categories and migration among them to investigate population structure and adaptation. en_US
dc.description.statementofresponsibility by Sarah Pacocha Preheim. en_US
dc.format.extent 183 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.rights.uri en_US
dc.subject Joint Program in Oceanography. en_US
dc.subject Civil and Environmental Engineering. en_US
dc.subject Woods Hole Oceanographic Institution. en_US
dc.subject.lcsh Marine microbiology en_US
dc.subject.lcsh Microbial diversity en_US
dc.title Ecology and population structure of vibrionaceae in the coastal ocean en_US
dc.type Thesis en_US Ph.D. en_US
dc.contributor.department Joint Program in Oceanography. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Civil and Environmental Engineering. en_US
dc.contributor.department Woods Hole Oceanographic Institution. en_US
dc.identifier.oclc 639534801 en_US

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