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Using design of experiments to improve a batch chemical process

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dc.contributor.advisor Roy Welsch and Charles L. Cooney. en_US Hill, Andrew, S.M. (Andrew James). Massachusetts Institute of Technology en_US
dc.contributor.other Leaders for Global Operations Program. en_US 2010-10-12T18:06:01Z 2010-10-12T18:06:01Z 2010 en_US 2010 en_US
dc.description Thesis (M.B.A.)--Massachusetts Institute of Technology, Sloan School of Management; and, (S.M.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering; in conjunction with the Leaders for Global Operations Program at MIT, 2010. en_US
dc.description Cataloged from PDF version of thesis. en_US
dc.description Includes bibliographical references (p. 70-71). en_US
dc.description.abstract Novartis Vaccines and Diagnostics has made a strong commitment to manufacturing seasonal influenza vaccines through their cell culture technology called Optaflu®. The goal of this project is to improve overall process yield by modifying the upstream process. The focus is on using a batch process to generate a high-density cell culture and then infecting said culture. This thesis presents the approach of using a Design of Experiment series to change a manufacturing process. Current vaccine production occurs with a fed-batch process by feeding glucose as a carbon-energy source for the final cell expansion step. This cell culture is diluted, infected, harvested, and purified for use in an influenza vaccine. Primarily, the project aims to increase cell density, using a batch process, at the infection step which should improve overall process yield. The project can therefore be broken into two main steps: batch cell growth and highdensity infection. Experiments for this project were conducted with a small-scale laboratory process that mimics the production process. The planned approach was a Design of Experiment series to screen parameters and partially optimize the cell growth process, a scale-up cell growth experiment, and finally another Design of Experiment series to explore high-density cell infection. While initial small-scale experiments showed extremely positive results, the results were not consistent and could not be replicated at a larger scale. A number of exploratory experiments were run to attempt to identify which factors inhibit high-density cell growth, particularly around scale-up, but no key parameter was identified. Given the process improvement and cost savings implications from the success of the initial small-scale experiments, this project is worth further exploration. en_US
dc.description.statementofresponsibility by Andrew Hill. en_US
dc.format.extent 71 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.rights.uri en_US
dc.subject Sloan School of Management. en_US
dc.subject Chemical Engineering. en_US
dc.subject Leaders for Global Operations Program. en_US
dc.title Using design of experiments to improve a batch chemical process en_US
dc.type Thesis en_US S.M. en_US M.B.A. en_US
dc.contributor.department Sloan School of Management. en_US
dc.contributor.department Massachusetts Institute of Technology. Dept. of Chemical Engineering. en_US
dc.contributor.department Leaders for Global Operations Program. en_US
dc.identifier.oclc 659806756 en_US

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