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Antibody-functionalized nanoporous surfaces enable high throughput specific cell capture

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dc.contributor.advisor Mehmet Toner. en_US Mittal, Sukant en_US
dc.contributor.other Harvard--MIT Program in Health Sciences and Technology. en_US 2012-09-13T19:01:44Z 2012-09-13T19:01:44Z 2012 en_US 2012 en_US
dc.description Thesis (Ph. D. in Medical and Electrical Engineering)--Harvard-MIT Program in Health Sciences and Technology, 2012. en_US
dc.description Cataloged from PDF version of thesis. en_US
dc.description Includes bibliographical references (p. 108-114). en_US
dc.description.abstract Adhesion-based cell capture on surfaces in microfluidic devices forms the basis of numerous biomedical diagnostics and in vitro assays. Solid surface microfluidic platforms have been widely explored for biomedical diagnostics since samples can be precisely and reproducibly manipulated under well-defined physicochemical conditions. However, at these small length scales, the fluid dynamics are dominated by the high surface-to-volume ratio and interfacial phenomena limiting device performance at high flow rates. In contrast, cell homing to porous vasculature is highly effective in vivo during inflammation; stem cell trafficking and cancer metastasis. In this work, we demonstrate that fluid-permeable surface functionalized with cell-specific antibodies can promote efficient and selective cell capture in vitro. This architecture might be advantageous due to enhanced transport due to fluid field modification leading to diverted streamlines towards the surface. Moreover, specific cell-surface interactions can be promoted due to reduced shear, allowing gentle cell rolling and arrest. Together, these synergistic effects enable highly effective target cell capture at flow rates over an order of magnitude larger than existing devices with solid surfaces. Additionally, in this study, we overcome a major limitation relevant to porous surfaces due to formation of stagnant layers of cells from non-target background population. These stagnant layers are detrimental to device performance as they act to reduce interaction of the cells with the reactive surface thereby reducing capture efficiency. We theoretically and experimentally understand the mechanisms for formation of the stagnant bioparticle layer in microfluidic devices and define a parameter space for optimal operation of the device over long periods of time. Key insights from these studies, collectively allow us to design a spatially modified microfluidic devices that allow us to isolate cancer lines as low as 5 cells/mL spiked into buffy coat. en_US
dc.description.statementofresponsibility by Sukant Mittal. en_US
dc.format.extent 114 p. en_US
dc.language.iso eng en_US
dc.publisher Massachusetts Institute of Technology en_US
dc.rights M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. en_US
dc.rights.uri en_US
dc.subject Harvard--MIT Program in Health Sciences and Technology. en_US
dc.title Antibody-functionalized nanoporous surfaces enable high throughput specific cell capture en_US
dc.title.alternative Nanoporous surfaces enable high throughput specific cell capture en_US
dc.type Thesis en_US Medical and Electrical Engineering en_US
dc.contributor.department Harvard--MIT Program in Health Sciences and Technology. en_US
dc.identifier.oclc 809078294 en_US

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