The Techniques and Discussion sessions provide an in-depth view of current technologies used in the biochemistry field that are briefly outlined in the lectures. Recitation class time focuses on technologies described in the methods sections of scientific papers that students are required to read. The table below includes an example set of notes taken by a student in the course for each Techniques and Discussion session (TD#).
| SES # | TOPICS |
|---|---|
| TD1 | Beta-ketoacyl-ACP Synthase I (FabB) (PDF) |
| TD2 | Smith Paper: Ranhan, V. S., A. K. Joshi, and S. Smith. "Mapping the Functional Topology of the Animal Fatty Acid Synthase." Biochemistry 40, no. 36 (2001): 10792-10799. TD class notes (PDF) |
| TD3 | Walsh Paper: Keating, T. A., and C. T. Walsh. "Initiation, Elongation, and Termination Strategies in Polyketide and Polypeptide Antibiotic Biosynthes." Current Opinion in Chemical Biology 3 (1999): 598-606. TD class notes (PDF) |
| TD4 | Endocytosis of LDL and Radioactivity Techniques (PDF) |
| TD5 | Structure (PDF) |
| TD6 | Hydroxyl Radical Footprinting (PDF) |
| TD7 | Gel Electrophoresis; Photoaffinity Probes (PDF) |
| TD8 | Rodnina Paper: Rodnina, M. V., T. Daviter, K. Gromadski, and W. Wintermeyer. Biochimie 84 (2002): 745-754. TD class notes (PDF) |
| TD9 | FRET, Steady State (PDF) |
| TD10 | Exam 3 Answers and Discussion |
| TD11 | GroEl / GroES (PDF) |
| TD12 | DnaJ Specificity (PDF) |