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An Ultra-Sensitive Step-Function Opsin for Minimally Invasive Optogenetic Stimulation in Mice and Macaques
Name
nihms-1582991.pdf
Description
Accepted version
Size
1.22 MB
Format
Adobe PDF
Checksum (MD5)
d523a759205f9ffd2aeac7991fda254f
Author(s)
Gong, Xin
Mendoza-Halliday, Diego
Ting, Jonathan T
Kaiser, Tobias
Sun, Xuyun
Bastos, André M
Wimmer, Ralf D
Guo, Baolin
Chen, Qian
Zhou, Yang
Date Issued
2020
Journal
Neuron
Publisher
Elsevier BV
Version
Author's final manuscript
Abstract
© 2020 Elsevier Inc. Optogenetics is among the most widely employed techniques to manipulate neuronal activity. However, a major drawback is the need for invasive implantation of optical fibers. To develop a minimally invasive optogenetic method that overcomes this challenge, we engineered a new step-function opsin with ultra-high light sensitivity (SOUL). We show that SOUL can activate neurons located in deep mouse brain regions via transcranial optical stimulation and elicit behavioral changes in SOUL knock-in mice. Moreover, SOUL can be used to modulate neuronal spiking and induce oscillations reversibly in macaque cortex via optical stimulation from outside the dura. By enabling external light delivery, our new opsin offers a minimally invasive tool for manipulating neuronal activity in rodent and primate models with fewer limitations on the depth and size of target brain regions and may further facilitate the development of minimally invasive optogenetic tools for the treatment of neurological disorders.
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
10.1016/J.NEURON.2020.03.032
