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dc.contributor.advisorScott R. Manalis.en_US
dc.contributor.authorChou, Nigel Shijieen_US
dc.contributor.otherMassachusetts Institute of Technology. Department of Biological Engineering.en_US
dc.date.accessioned2017-12-05T19:15:31Z
dc.date.available2017-12-05T19:15:31Z
dc.date.copyright2017en_US
dc.date.issued2017en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/112498
dc.descriptionThesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2017.en_US
dc.descriptionCataloged from PDF version of thesis.en_US
dc.descriptionIncludes bibliographical references (pages 117-119).en_US
dc.description.abstractThe increased precision offered by developments in suspended microchannel resonator (SMR) technology opens the possibility for measuring small mass changes in cells. Mass accumulation rate (MAR) measurements in single suspended cells over short periods of time have the potential for characterizing heterogeneous collections of tumorigenic cells and serve as a functional marker for the effects of anti-cancer drugs. In this thesis we adapt mass accumulation measurements for use in Glioblastoma Multiforme (GBM) patient-derived cell lines, exploring the heterogeneity between and within patient tumors, and validating the measurement as a predictor of drug susceptibility with response times on the order of 24 to 48 hours using an experimental MDM2 inhibitor. While MAR measurements can be performed on suspended single cells with high precision, it has not yet been adapted for measuring the growth of adherent cells. We develop a technique to measure mass accumulation in cells adhered to the inner surface of the resonator channel. To overcome challenges inherent in such a measurement, we use infrared imaging and multiple resonant modes to reveal the cell's position in the SMR, and utilize differential measurements from a second cantilever to account for frequency drift.en_US
dc.description.statementofresponsibilityby Nigel Shijie Chou.en_US
dc.format.extent119 pagesen_US
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsMIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582en_US
dc.subjectBiological Engineering.en_US
dc.titleMeasuring mass changes in single suspended and adherent cells, with applications to personalized medicine in Glioblastoma Multiforme (GBM)en_US
dc.typeThesisen_US
dc.description.degreePh. D.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineering.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineering
dc.identifier.oclc1011521056en_US


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