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Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms
(Massachusetts Institute of Technology, 2006)
Optical tweezers instruments use laser radiation pressure to trap microscopic dielectric beads. With the appropriate chemistry, such a bead can be attached to a single molecule as a handle, permitting the application of ...
DNA alkylation repair deficient mice are susceptible to chemically induced Inflammatory Bowel Disease
(Massachusetts Institute of Technology, 2006)
The two most common forms of inflammatory bowel disease (IBD) are ulcerative colitis (UC) and Crohn's Disease (CD), which affect more than 1 million Americans. Recently the incidence of IBD has been rising in Japan, Europe ...
Optimization of cell adhesion environments for a liver cell bioreactor
(Massachusetts Institute of Technology, 2006)
The MilliF bioreactor offers great potential for the formation of i vivo-like liver tissue outside the body, making it a valuable tool for applications such as drug toxicity models and biosensors. Cell adhesion is an ...
Investigating the role of calcium in the biomechanical response of neutrophils to mechanical deformation experienced in the pulmonary capillaries
(Massachusetts Institute of Technology, 2006)
Neutrophils in the pulmonary microcirculation are subjected to mechanical deformation while traveling through capillaries of sizes much smaller than the mean neutrophil diameter. This deformation has been shown to result ...
Regulation of specific connexins differentially alters gap junction permeability and endothelial cell function
(Massachusetts Institute of Technology, 2006)
While many have explored how vascular processes alter gap junction communication and composition few have analyzed the role of specific gap junction connexin proteins in regulating cellular communication and wound healing. ...
A novel high-throughput in-cell Western assay for the quantitative measurement of signaling dynamics in DNA damage signaling networks : cell decision processes in response to DNA double strand breaks
(Massachusetts Institute of Technology, 2006)
Following exposure to DNA damage, cells initiate a stress response involving multiple protein kinase signaling cascades. The DNA damage response results in one of several possible cell-fate decisions, or cellular responses: ...
Synthesis and error correction methods in gene fabrication
(Massachusetts Institute of Technology, 2006)
Gene Fabrication technology involves the development and optimization of methods relevant to the in vitro synthesis of any given target gene sequence(s) in the absence of template. The driving purpose of this field of ...
Optimization of organelle fractionation methods for quantitative analysis of gene delivery trafficking kinetics
(Massachusetts Institute of Technology, 2006)
Nonviral vector research and development has been stunted by a lack of knowledge and understanding of how vectors are trafficked within the cell. Research currently involves mass screenings of different combinations of ...
Towards a microfluidic disease detection deviced based on cellular adhesion differences
(Massachusetts Institute of Technology, 2006)
There is a great need in the fields of biology, medicine, and pharmaceuticals to create high-throughput devices for the detection of specific cell states in a heterogeneous mixture of cells. The desire is to differentiate ...