Principles of Nanoparticle Design for Genome Editing in Plants
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fgeed-04-846624.pdf
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1.03 MB
Format
Adobe PDF
Checksum (MD5)
2d65fbba228ac2555845ad0973901111
Author(s)
Sharma, Pushkal
Thomas, Tedrick
Lew, Salim
Matos, Juliana
Wang, Yanpeng
Date Issued
March 7, 2022
Journal
Frontiers in Genome Editing
Publisher
Frontiers Media SA
Citation
Sharma P and Lew TTS (2022) Principles of Nanoparticle Design for Genome Editing in Plants. Front. Genome Ed. 4:846624.
Version
Final published version
Abstract
Precise plant genome editing technologies have provided new opportunities to accelerate crop improvement and develop more sustainable agricultural systems. In particular, the prokaryote-derived CRISPR platforms allow precise manipulation of the crop genome, enabling the generation of high-yielding and stress-tolerant crop varieties. Nanotechnology has the potential to catalyze the development of a novel molecular toolbox even further by introducing the possibility of a rapid, universal delivery method to edit the plant genome in a species-independent manner. In this Perspective, we highlight how nanoparticles can help unlock the full potential of CRISPR/Cas technology in targeted manipulation of the plant genome to improve agricultural output. We discuss current challenges hampering progress in nanoparticle-enabled plant gene-editing research and application in the field, and highlight how rational nanoparticle design can overcome them. Finally, we examine the implications of the regulatory frameworks and social acceptance for the future of nano-enabled precision breeding in the developing world.
Subjects
nanotechnology
gene editing
precision breeding
nanoparticles
CRISPR
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3389/fgeed.2022.846624
