Design and fabrication of a microfluidies gradient generator system for high-throughput molecular interaction studies
Author(s)Chen, Guan-Jong, 1981-
Massachusetts Institute of Technology. Biological Engineering Division.
Robert S. Langer.
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Design and fabrication of a microfluidics system capable of generating reproducible and controlled micro-biochemical environments that can be used as a diagnostic assay and microreactor is important. Here, a simple technique was developed to create a robust microfluidics system capable of generating precise gradients of biochemical properties within its channels. Through this approach, it is possible to create a gradient generator with mammalian cells patterned and seeded under its poly(dimethylsiloxane) (PDMS) channels. Cells that were seeded and patterned under the PDMS channels remained viable and capable of performing intracellular reactions. Using the gradient generator within the PDMS microfluidic device, a gradient of specific and controlled biochemicals can be flowed on seeded cells allowing for high-throughput molecular interaction analysis. The microfluidics system provides a way to study and analyze cell response in the presence of a combination of biochemical signals.
Thesis (S.M. in Toxicology)--Massachusetts Institute of Technology, Biological Engineering Division, 2004.Includes bibliographical references (leaves 45-47).
DepartmentMassachusetts Institute of Technology. Biological Engineering Division.; Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology
Biological Engineering Division.