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dc.contributor.advisorRobert S. Langer.en_US
dc.contributor.authorChen, Guan-Jong, 1981-en_US
dc.contributor.otherMassachusetts Institute of Technology. Biological Engineering Division.en_US
dc.date.accessioned2005-06-02T19:44:57Z
dc.date.available2005-06-02T19:44:57Z
dc.date.copyright2004en_US
dc.date.issued2004en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/18043
dc.descriptionThesis (S.M. in Toxicology)--Massachusetts Institute of Technology, Biological Engineering Division, 2004.en_US
dc.descriptionIncludes bibliographical references (leaves 45-47).en_US
dc.description.abstractDesign and fabrication of a microfluidics system capable of generating reproducible and controlled micro-biochemical environments that can be used as a diagnostic assay and microreactor is important. Here, a simple technique was developed to create a robust microfluidics system capable of generating precise gradients of biochemical properties within its channels. Through this approach, it is possible to create a gradient generator with mammalian cells patterned and seeded under its poly(dimethylsiloxane) (PDMS) channels. Cells that were seeded and patterned under the PDMS channels remained viable and capable of performing intracellular reactions. Using the gradient generator within the PDMS microfluidic device, a gradient of specific and controlled biochemicals can be flowed on seeded cells allowing for high-throughput molecular interaction analysis. The microfluidics system provides a way to study and analyze cell response in the presence of a combination of biochemical signals.en_US
dc.description.statementofresponsibilityby Guan-Jong Chen.en_US
dc.format.extent47 leavesen_US
dc.format.extent2882891 bytes
dc.format.extent2886580 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.language.isoengen_US
dc.publisherMassachusetts Institute of Technologyen_US
dc.rightsMIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.en_US
dc.rights.urihttp://dspace.mit.edu/handle/1721.1/7582
dc.subjectBiological Engineering Division.en_US
dc.titleDesign and fabrication of a microfluidies gradient generator system for high-throughput molecular interaction studiesen_US
dc.typeThesisen_US
dc.description.degreeS.M.in Toxicologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Biological Engineering Division.en_US
dc.identifier.oclc57351170en_US


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