DNA Directed Assembly Probe for Detecting DNA-Protein Interaction in Microarray Format
Author(s)Ng, Jin Kiat; Ajikumar, Parayil Kumaran; Tang, Yew Chung; Stephanopoulos, Gregory; Too, Heng-Phon
Quantifying DNA-protein interaction using DNA microarrays are gaining increasing attention due to their ability to profile specificity of interactions in a high-throughput manner. This paper describes a new approach that used the ability of ssDNA-dsDNA probe to complex with DNA binding proteins in the solution phase and then spatially immobilized onto microarray through specific DNA hybridization. In one case, the Spatially Addressable DNA Array (SADA) approach demonstrated that enzymatic cleavage in solution is more efficient than if conducted heterogeneously. In addition, binding of RNA polymerase with promoter DNA could be detected with this strategy.
Chemical and Pharmaceutical Engineering (CPE)
DNA-Protein Interaction, DNA Directed Assembly, Restriction Enzyme, RNA Polymerase, ssDNA-dsDNA Conjugate