Now showing items 1-4 of 4

    • Cloning Standard for Mammalian BioBrick Parts and Devices 

      Bartoschek, Michael; Mugahid, Douaa; Rademacher, Anne; Meyer, Hannah; Velten, Lars; e.a. (2009-10-21)
      To introduce a common cloning standard for BioBrick parts that find application in mammalian cells.
    • HiCT: High Throughput Protocols For CPE Cloning And Transformation 

      Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikolaos; Kats, Ilia; e.a. (2013-10-04)
      The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High ...
    • RA-PCR, a method for the generation of randomized promoter libraries 

      Velten, Lars; Haas, Simon; Rademacher, Anne; Meyer, Hannah; Eils, Roland (2009-10-21)
      The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ...
    • Standard for Synthesis of Customized Peptides by Non-Ribosomal Peptide Synthetases 

      Beer, Ralf; Christiansen, Tania; Herbst, Konrad; Ignatiadis, Nikos; Kats, Ilia; e.a. (2013-10-04)
      The purpose of this RFC is to introduce a standardized framework for the engineering of customizable non-ribosomal peptide synthetases (NRPS) and their application for in vivo and in vitro synthesis of short non-ribosomal ...