BBF RFC 38: RFC 38: Building Blocks - Standard Large DNA/Genome Construction

Abstract

The physical assembly of standard parts is currently a non-standard process, which can either come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers, or overlap assembly PCR. Furthermore, current Biobrick construction standards [RFC 10,11,12 etc.] rely heavily on restriction enzyme based methods, which can be sequence, cost and time restrictive. This is especially true of large DNA assembly, where 100% control of over DNA sequence may be mandatory, such as in whole genome assembly. We propose a novel standard, The Building Block Method, for both the construction of standard parts and their assembly This Building Block standard can be either A) an interchangeable or B) a non-interchangeable format, based on the desired use of the part.