Browsing MIT Open Access Articles by Author "Yachie, Nozomu"

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    • Engineered CRISPR-Cas9 nuclease with expanded targeting space 

      Nishimasu, Hiroshi; Shi, Xi; Ishiguro, Soh; Gao, Linyi; Hirano, Seiichi; e.a. (American Association for the Advancement of Science (AAAS), 2018-08)
      The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genome-editing tool. However, the widely used Streptococcus pyogenes Cas9 enzyme (SpCas9) requires an NGG protospacer adjacent motif (PAM) for target ...