| dc.contributor.author | Ellison, Michael | |
| dc.contributor.author | Ridgway, Doug | |
| dc.contributor.author | Fedor, Justin | |
| dc.contributor.author | Garside, Erin | |
| dc.contributor.author | Robinson, Kelly | |
| dc.contributor.author | Lloyd, David | |
| dc.date.accessioned | 2009-10-29T23:42:34Z | |
| dc.date.available | 2009-10-29T23:42:34Z | |
| dc.date.issued | 2009-10-29T23:42:34Z | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/49518 | |
| dc.description.abstract | The BioBytes assembly method provides a new high-throughput way of assembling
plasmids as well as other large DNA constructs. Using a bead-linked assembly of genetic
parts with long (12 bp) standardized sticky ends, it allows for greater speed and
efficiency in assembling these components. This document defines a standard for genetic
parts compatible with the assembly system and describes detailed protocols for the
construction of such parts and their rapid assembly. Although the original motivation for
the method was to produce whole synthetic genomes, the design allows for any type of
DNA construction. | en |
| dc.language.iso | en_US | en |
| dc.relation.ispartofseries | BBF RFC;47 | |
| dc.subject | synthetic biology | en |
| dc.subject | standard biological parts | en |
| dc.title | BBF RFC 47: BioBytes Assembly Standard | en |
| dc.type | Technical Report | en |
