Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR
Author(s)
Olchowik, Anna; Lower, Michal; Moreno, Cherry; Pankowski, Jaroslaw
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The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, and therefore it is can be expressed in PoPS [1]
Date issued
2010-12-05Series/Report no.
BBF RFC;58
Keywords
measurement
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