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Promoter directionality is controlled by U1 snRNP and polyadenylation signals in mouse embryonic stem cells
(Massachusetts Institute of Technology, 2013)
RNA polymerase II (RNAPII) transcription is a tightly regulated process controlling cell type and state. Advancements in our understanding of how transcription is regulated will provide insight into the mechanisms controlling ...
Spatial and temporal coordination of genome segregation with activation of the Mitotic Exit Network
(Massachusetts Institute of Technology, 2012)
In budding yeast, an essential Hippo-like signal transduction cascade known as the Mitotic Exit Network (MEN) governs the final cell cycle transition, the mitosis to G1 transition. To ensure the accurate execution of ...
Modulation of host NF-[k̳̳a̳p̳p̳a̳]B pathway by the Toxoplasma gondii secreted factor, GRA15
(Massachusetts Institute of Technology, 2013)
The apicomplexan protozoan Toxoplasma gondii is an obligate intracellular pathogen that infects all warm blooded animals, including nearly thirty percent of the human population worldwide. Toxoplasma's success as a parasite ...
Identification and Characterization of Modulators of Chemotherapeutic Response in Mouse Models of Cancer
(Massachusetts Institute of Technology, 2010)
Chemotherapeutic drug resistance is a major cause of cancer treatment failure. While much attention has been focused on the genetics of tumor development, less is known about the genetic determinants of therapeutic outcome. ...
Molecular titration by MicroRNAs and target mimic inhibitors
(Massachusetts Institute of Technology, 2010)
MicroRNAs (miRNAs) are short, highly conserved non-coding RNA molecules that repress gene expression in a sequence-dependent manner. Each miRNA is predicted to target hundreds of genes, and a majority of protein-coding ...
Functional connectivity of coral reef fishes in a tropical seascape assessed by compound-specific stable isotope analyses
(Massachusetts Institute of Technology, 2011)
The ecological integrity of tropical habitats, including mangroves, seagrass beds and coral reefs, is coming under increasing pressure from human activities. Many coral reef fish species are thought to use mangroves and ...
The Importance of RNA Pairing Stability and Target Concentration for Regulation by MicroRNAs
(Massachusetts Institute of Technology, 2012)
Regulation of gene expression in eukaryotes is highly precise and complex. Changes in expression can define the fate of each cell, convert healthy tissues to diseased ones, and even lead to speciation. Regulation occurs ...
In vivo pool-based shRNA screens to identify modulators of disease progression in hematopoietic malignancies
(Massachusetts Institute of Technology, 2012)
shRNA screens have been very effective in identifying novel cancer genes in mammalian cells, but they have primarily been limited to in vitro applications in tumor cell lines. Whereas in vivo retroviral mutagenesis screens ...
The cell-secreted microenvironment : shaping embryonic stem cell self-renewal and differentiation
(Massachusetts Institute of Technology, 2012)
The objective of this work is to obtain an in depth understanding of how embryonic stem cell-secreted signals contribute to their identity. We analyze the contribution of broad and specific signals present in the cell-secreted ...
Protein quality control in the mammalian endoplasmic reticulum
(Massachusetts Institute of Technology, 2011)
Quality control is an important part of protein biogenesis. Aberrant proteins must be destroyed before they aggregate and cause deleterious effects. Failure to do so can result in cell death or malfunction and, ultimately, ...