Browsing Synthetic Biology by Title
Now showing items 100-119 of 139
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Open licensing of BioBrick parts
(2010-12-05)This document provides recommendations for licensing of community-created biological parts, especially in BioBrick standard. -
Open Sequence Initiative: a part submission standard to complement modern DNA assembly techniques
(2013-10-06)The discipline of synthetic biology emphasizes the application of engineering principles such as standardization, abstraction, modularity, and rational design to complex biological systems. The archetypical example of such ... -
pCH1497-ASD1
(2008-05-23) -
PCR - Ligation Assembly Standard for BioBrick Parts
(2011-12-15)This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This ... -
Power and responsibility
(2006-11-21)Peter Parker and Uncle Ben are on my mind. The reason is that is that a month ago I was jumped by Craig Venter. There were TV cameras around. The live audience was an interesting, edgy mix, on the interface between ... -
A Practical Perspective on DNA Synthesis and Biological Security (12/4/2006 Draft)
(2006-12-12)Few developments have leapfrogged over predecessor technology as quickly and extensively as synthetic biology. Based on cutting-edge DNA synthesis technology, synthetic biology has already fueled an expansion of ... -
Promoter and Coding Sequence Considerations for Caenorhabditis elegans and Other Eukaryotes
(2010-11-07)Our primary aim is to provide a standard format for protein-coding BioBricks intended for eukaryotes, especially in the nematode worm Caenorhabditis elegans, so that future teams will be able to correctly take advantage ... -
Proposed Conceptual Guidelines for the Design of a BioBrick Graphical Language & an Example
(2009-03-12)As we update the current BioBrick symbols we have an opportunity to look at the end users of a BioBrick Graphical language and develop design guidelines so that the new symbols best suit their needs. This document explores ... -
Provisional BioBrick Language (PoBoL)
(2009-05-15)This BioBricks Foundation Request for Comments (BBF RFC) describes a semantic markup language for publishing and sharing information about BioBricks on the World Wide Web. This BBF RFC includes the recommendation for the ... -
Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry
(2010-10-31)Purpose of this RFC is to provide method of measurement of bacterial invasion into mammalian cells. Also this RFC defines Percent of INvasion (PIN) unit. -
RA-PCR, a method for the generation of randomized promoter libraries
(2009-10-21)The purpose of this RFC is to provide instructions for the synthesis of promoters in mammalian cells that are active at a desired cellular condition (where a cellular condition is specified by the activity of a set of ... -
Rapid Characterization of Cellular Pathways Using Time-Varying Signals
(International Conference on Systems Biology, 2005-10-21)The use of traditional tools for the discovery and characterization of biological systems has resulted in a wealth of biological knowledge. Unfortunately, only a small portion of the biological world is well-understood ... -
Raw Data, Win MN, Smolke CD. 2008. Higher-order cellular information processing with synthetic RNA devices. Science. 322: 456-60. DOI: 10.1126/science.1160311
(2009-07-29)This supplement provides additional detail on the data analysis methods and the raw data for the RNA devices presented in the published manuscript and supporting online material. The information provided in the current ... -
Recombination Based Part Assembly
(2010-12-05)Here we propose a new recombination-based assembly standard, optimized to allow for efficient cloning of mammalian vectors. -
Refactoring Bacteriophage T7 (2004 version)
(2004-10)Natural biological systems are selected by evolution to continue to exist. Evolution might give rise to complicated systems that are difficult to discover, measure, model, and direct. Here, we redesign the genome of a ... -
Registry of BioBricks Models using CellML
(2007-10-04)One of the main goals in Synthetic Biology is to assess the feasibility of building novel biological systems from interchangeable and standardized parts. In order to collect and share parts, a Registry of standardized DNA ... -
A RESTful API for Supporting Automated BioBrick Model Assembly
(2010-12-05)Constructing simulatable models for BioBricks by hand is a complex and time-consuming task. The time taken could be reduced by using Computer Aided Design (CAD) tools to aid in designing models, but these tools need to be ... -
Reusable rapid assembly of genetic parts for Neurospora crassa
(2011-10-17)This BBF RFC provides a method in which standardized parts can be easily created for Neurospora crassa. -
Risk Assessment of Synthetic Genomics: A Biosafety and Biosecurity Perspective
(2007-12-04)The ability to synthesize molecules found in living organisms is not new for scientists in the fields of biochemistry and molecular biology. However, the “synthetic biology” made possible by the genetic mapping of ...